Multiple Reassorted Viruses as Cause of Highly Pathogenic Avian Influenza A(H5N8) Virus Epidemic, the Netherlands, 2016.
Multiple Reassorted Viruses as Cause of Highly Pathogenic Avian Influenza A(H5N8) Virus Epidemic, the Netherlands, 2016.
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DOI：
10.3201/eid2312.171062
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发表时间：
2017-12
影响因子：
11.8
通讯作者：
Koch G
中科院分区：
文献类型：
--
作者：
Beerens N;Heutink R;Bergervoet SA;Harders F;Bossers A;Koch G
2016年，荷兰爆发H5N8亚型高致病性禽流感病毒疫情，导致野生鸟类大量死亡，多个商业家禽养殖场和圈养鸟类受到影响。我们进行了全基因组测序，以研究野鸟和家禽病毒之间的关系。系统发育分析显示，这些病毒与2016年5月在俄罗斯检测到的H5进化枝2.3.4.4病毒相关，但含有新的聚合酶碱性2和核蛋白基因片段以及聚合酶酸性片段的2种不同变体。分子年代测定表明，这些基因重组事件很可能发生在俄罗斯或蒙古的野生鸟类身上。此外，在荷兰的野生鸟类中检测到2种遗传上不同的H5N5抗病毒。我们的研究为H5进化枝2.3.4.4病毒的快速和持续重配提供了证据，这可能导致病毒特征的快速变化，如致病性，感染性，传播性和人畜共患病潜力。
In 2016, an epidemic of highly pathogenic avian influenza A virus subtype H5N8 in the Netherlands caused mass deaths among wild birds, and several commercial poultry farms and captive bird holdings were affected. We performed complete genome sequencing to study the relationship between the wild bird and poultry viruses. Phylogenetic analysis showed that the viruses are related to H5 clade 2.3.4.4 viruses detected in Russia in May 2016 but contained novel polymerase basic 2 and nucleoprotein gene segments and 2 different variants of the polymerase acidic segment. Molecular dating suggests that the reassortment events most likely occurred in wild birds in Russia or Mongolia. Furthermore, 2 genetically distinct H5N5 reassortant viruses were detected in wild birds in the Netherlands. Our study provides evidence for fast and continuing reassortment of H5 clade 2.3.4.4 viruses, which might lead to rapid changes in virus characteristics, such as pathogenicity, infectivity, transmission, and zoonotic potential.