Recombinants in the H-2S/H-2D interval of mouse chromosome 17 define the map position of a gene for cleft palate susceptibility.
Recombinants in the H-2S/H-2D interval of mouse chromosome 17 define the map position of a gene for cleft palate susceptibility.
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DOI：
10.1002/tera.1420380605
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发表时间：
1988-12
期刊：
影响因子：
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通讯作者：
David L. Gasser;K. Yadvish;Michele A. Trammell;Allen S. Goldman
中科院分区：
文献类型：
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作者：
David L. Gasser;K. Yadvish;Michele A. Trammell;Allen S. Goldman
已知小鼠17号染色体的H-2区域包括一个或多个影响可的松诱导的腭裂易感性的基因。我们现在已经研究了在H-2S和H-2D之间的间隔中具有交叉的同源菌株，并且已经观察到被认为具有相同H-2单倍型的重组体之间的易感性的显著差异。妊娠小鼠在妊娠第11 - 14天注射100 mg可的松/kg体重。B10.A（2 R）中腭裂的频率显著高于B10.A（1 R），尽管两者在S和D基因座之间的间隔中具有H-2a/H-2b交叉，并且在先前表征的所有基因座上具有相同的等位基因。B10.BAR5和B10.BAR12都比B10.A（18 R）明显更易感，尽管这些菌株在先前表征的所有基因座上也具有相同的等位基因。所有这三种菌株都具有H-2b/H-2a重组染色体，在S/D间隔中具有交叉。通过对回交后代H-2纯合子的检测，证实了H-2与B10.BAR5高感基因的遗传连锁。因此，这些数据表明，编码易感性的基因，我们命名为Cps-1，映射在H-2S和H-2D之间的350 kb间隔，我们发现不同的同源菌株在此间隔内具有不同的交叉点。Cps-1位点的等位基因具有胚胎效应，但对母体环境没有明显的影响。
The H-2 region of mouse chromosome 17 is known to include one or more genes that affect susceptibility to cortisone-induced cleft palate. We have now studied congenic strains that possess crossovers in the interval between H-2S and H-2D and have observed significant differences in susceptibility among recombinants that had been believed to possess the same H-2 haplotypes. Pregnant mice were injected on days 11 through 14 of gestation with 100 mg of cortisone per kg of body weight. The frequency of cleft palate in B10.A(2R) was significantly greater than in B10.A(1R), despite the fact that both have H-2a/H-2b crossovers in the interval between the S and D loci and have the same alleles at all loci that have been previously characterized. Both B10.BAR5 and B10.BAR12 were significantly more susceptible than B10.A(18R), although these strains also share the same alleles at all loci that have been previously characterized. All three of these strains have H-2b/H-2a recombinant chromosomes, with crossovers in the S/D interval. Genetic linkage between H-2 and the high-susceptibility gene of B10.BAR5 was confirmed by testing H-2 homozygotes derived by intercrossing backcross animals. These data therefore suggest that a gene coding for susceptibility, which we designate Cps-1, maps in the 350-kb interval between H-2S and H-2D, and the congenic strains that we have found to be different have different crossover points within this interval. Alleles at the Cps-1 locus have embryonic effects, but no demonstrable effects on the maternal environment.