Math1-driven GFP expression in the developing nervous system of transgenic mice
Math1-driven GFP expression in the developing nervous system of transgenic mice
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DOI：
10.1016/s1567-133x(03)00089-9
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发表时间：
2003-08-01
影响因子：
1.2
通讯作者：
Johnson, JE
中科院分区：
文献类型：
--
作者：
Lumpkin, EA;Collisson, T;Johnson, JE
Math 1是一种bHLH转录因子，在神经系统多个区域的神经祖细胞中表达。之前我们发现了一个Math 1增强子，它以Math 1的特定模式指导报告基因的表达[Development 127(2000) 1185]。我们已经使用该增强子的一部分在转基因小鼠的Math 1谱系中驱动核GFP报告基因的表达。在这个转基因小鼠品系中，GFP在Math 1结构域中表达(1)发育中的脊髓到背侧中间神经元的祖细胞，(2)发育中的小脑的颗粒细胞祖细胞，(3)皮肤中的默克尔细胞，(4)发育中的前庭和听觉系统的毛细胞。此外，检测到非math 1相关的表达，这可能是由于缺乏来自转基因的抑制调控序列。这些表达域包括(1)发育中的肢体的顶端外胚层嵴，(2)发育中的皮层和脊髓的有丝分裂后细胞，(3)齿状回，(4)视网膜，(5)嗅觉上皮。由于GFP标记了活组织中特定的神经元细胞类型，因此该转基因菌株为未来研究中枢神经系统和感觉系统中不同类型细胞的发育和电生理特性提供了有力的工具。(C) 2003 Elsevier Science B.V.版权所有
Math 1 is a bHLH transcription factor expressed in neural progenitor cells in multiple regions of the nervous system. Previously we identified a Math 1 enhancer that directs expression of reporter genes in a Math 1 specific pattern [Development 127 (2000) 1185]. We have used a portion of this enhancer to drive expression of a nuclear GFP reporter in the Math 1 lineage in transgenic mice. In this transgenic mouse strain, GFP is expressed in Math 1 domains in the (1) developing spinal cord in progenitors to dll dorsal interneurons, (2) granule-cell progenitors in the developing cerebellum, (3) Merkel cells in the skin, and (4) hair cells in the developing vestibular and auditory systems. Furthermore, non-Math 1 related expression is detected that is likely due to the absence of inhibitory regulatory sequences from the transgene. These expression domains include (1) the apical ectodermal ridge in developing limbs, (2) post-mitotic cells in the developing cortex and spinal cord, (3) the dentate gyrus, (4) retina, and (5) olfactory epithelium. Because GFP marks specific neuronal cell types in living tissue, this transgenic strain is a powerful tool for future studies on the development and electrophysiological properties of distinct cell types in the central nervous system and in sensory systems. (C) 2003 Elsevier Science B.V. All rights reserved.