Mitochondria-targeting Au nanoclusters enhance radiosensitivity of cancer cells
Mitochondria-targeting Au nanoclusters enhance radiosensitivity of cancer cells
复制标题
线粒体靶向金纳米团簇增强癌细胞的放射敏感性
DOI：
10.1039/c7tb00422b
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发表时间：
2017-06-14
影响因子：
7
通讯作者：
Liu, Jian
中科院分区：
文献类型：
--
作者：
Fang, Xu;Wang, Yaling;Liu, Jian
放射治疗是临床治疗癌症的重要技术，但放射线照射后患者会产生严重的副作用。目前迫切需要开发具有良好成像能力和有效放射增敏作用的治疗诊断剂，以最大限度地减少X射线照射。在此，我们报告了一种方法来合成肽模板Au纳米簇（AuNCs）的治疗诊断放射增敏。设计了一种新的多肽（CCYKFR）用于制备具有均匀尺寸分布和高光稳定性的荧光（656 nm）的AuNCs。CCYKFR-AuNC的特征在于内吞作用后在线粒体上的高效靶向/积累。通过一系列的实验，我们证明了4戈伊X射线照射的CCYKFR-AuNC可以引入线粒体ROS的爆发和严重的DNA损伤，导致癌细胞死亡。这项研究提出了一个重要的战略，设计治疗诊断纳米材料，提高放射增敏的发展新的抗癌疗法。
fRadiotherapy is an important technology for the clinical treatment of cancer, but the patients suffer from the severe side effects after exposure to radiation. There is an urgent need to develop theranostic agents with excellent imaging capability and effective radiosensitization in order to minimize X-ray irradiation. Herein, we report an approach to synthesize peptide-templated Au nanoclusters (AuNCs) for theranostic radiosensitization. A new peptide (CCYKFR) is designed for the preparation of AuNCs with uniform size distribution and fluorescence (656 nm) of high photostability. CCYKFR-AuNCs feature highly efficient targeting/accumulation on mitochondria after endocytosis. With a series of experiments, we demonstrate that CCYKFR-AuNCs irradiated by 4 Gy X-rays can introduce a burst of mitoROS and severe DNA damage leading to cancer cell death. This study presents an important strategy to design theranostic nanomaterials with improved radiosensitization for the development of new anti-cancer therapies.