Potential Phosphorus Uptake Mechanisms in the Deep Sedimentary Biosphere
Potential Phosphorus Uptake Mechanisms in the Deep Sedimentary Biosphere
复制标题
深层沉积生物圈中潜在的磷吸收机制
DOI：
10.3389/fmars.2022.907527
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发表时间：
2022-06
期刊：
影响因子：
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通讯作者：
D. Defforey;B. Tully;J. Sylvan;B. Cade‐Menun;Brandi Kiel Reese;L. Zinke;A. Paytan
中科院分区：
文献类型：
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作者：
D. Defforey;B. Tully;J. Sylvan;B. Cade‐Menun;Brandi Kiel Reese;L. Zinke;A. Paytan
我们对深海底环境中磷（P）动态的了解仍然有限。在这里，我们调查潜在的微生物磷的吸收机制，在贫营养海洋沉积物下的北大西洋环流和它们的有机磷化合物的相对分布的影响，作为一个功能的埋藏深度和不断变化的氧化还原条件。我们使用宏基因组分析，以确定是否存在微生物的功能基因有关的P吸收和代谢，和解决方案31 P核磁共振光谱（31 P NMR），以表征和量化P基板。用31P NMR鉴定的磷化合物或化合物类别包括无机P化合物（正磷酸盐、焦磷酸盐、多磷酸盐）、膦酸盐、正磷酸单酯（包括肌醇六磷酸立体异构体）和正磷酸二酯（包括DNA和磷脂降解产物）。一些基因包括与磷酸盐转运、磷酸盐和多磷酸盐代谢以及亚磷酸盐摄取相关的基因。我们的研究结果表明，深层沉积生物圈可能已经适应了利用多种磷底物，并且可能在肌醇和糖磷酸盐以及还原的磷化合物和多磷酸盐的逐渐分解中发挥作用。
Our understanding of phosphorus (P) dynamics in the deep subseafloor environment remains limited. Here we investigate potential microbial P uptake mechanisms in oligotrophic marine sediments beneath the North Atlantic Gyre and their effects on the relative distribution of organic P compounds as a function of burial depth and changing redox conditions. We use metagenomic analyses to determine the presence of microbial functional genes pertaining to P uptake and metabolism, and solution 31P nuclear magnetic resonance spectroscopy (31P NMR) to characterize and quantify P substrates. Phosphorus compounds or compound classes identified with 31P NMR include inorganic P compounds (orthophosphate, pyrophosphate, polyphosphate), phosphonates, orthophosphate monoesters (including inositol hexakisphosphate stereoisomers) and orthophosphate diesters (including DNA and phospholipid degradation products). Some of the genes identified include genes related to phosphate transport, phosphonate and polyphosphate metabolism, as well as phosphite uptake. Our findings suggest that the deep sedimentary biosphere may have adapted to take advantage of a wide array of P substrates and could play a role in the gradual breakdown of inositol and sugar phosphates, as well as reduced P compounds and polyphosphates.