Average protein density is a molecular-weight-dependent function
Average protein density is a molecular-weight-dependent function
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DOI：
10.1110/ps.04688204
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发表时间：
2004-10-01
期刊：
影响因子：
8
通讯作者：
Craievich, AF
中科院分区：
文献类型：
--
作者：
Fischer, H;Polikarpov, I;Craievich, AF
蛋白质的质量密度是一个相关的基本生物物理量。它也是一个有用的输入参数，例如，通过蛋白质结晶学确定三维结构和通过分析超速离心法研究溶液中的蛋白质低聚物。我们对已发表的关于这个问题的理论和实验研究进行了批判性的分析，得出的结论是蛋白质的平均密度并不像通常假设的那样是一个常数。对于相对分子质量小于20 kDa的蛋白质，平均密度呈现正偏差，且随相对分子质量的减小而增大。提出了一个简单的依赖于分子量的函数，它提供了对平均蛋白质密度的更准确的估计。
The mass density of proteins is a relevant basic biophysical quantity. It is also a useful input parameter, for example, for three-dimensional structure determination by protein crystallography and studies of protein oligomers in solution by analytic ultracentrifugation. We have performed a critical analysis of published, theoretical, and experimental investigations about this issue and concluded that the average density of proteins is not a constant as often assumed. For proteins with a molecular weight below 20 kDa, the average density exhibits a positive deviation that increases for decreasing molecular weight. A simple molecular-weight-depending function is proposed that provides a more accurate estimate of the average protein density.