Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana
Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana
复制标题
烟草种间体细胞融合后含有混合和分选叶绿体的细胞杂种
DOI：
10.1007/bf00276566
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发表时间：
1983
影响因子：
5.4
通讯作者：
E. Galun
中科院分区：
文献类型：
--
作者：
R. Fluhr;D. Aviv;M. Edelman;E. Galun
利用烟叶（SR-1）原生质体、烟叶（细胞质雄性不育系92）原生质体和烟叶（sylvestris）原生质体“供体-受体”融合转移链霉素抗性。结果表明，链霉素抗性（SR-1）是一个独立于线粒体标记分离的叶绿体标记。在融合实验中，以烟草（92行）为受体，在链霉素存在下镀微愈伤组织。在这种情况下，叶绿体分选发生在植株再生之前的一个阶段，产生稳定的链霉素抗性杂交体。在以烟叶为受体的融合中，没有进行链霉素耐药的直接筛选。在这种情况下，叶绿体分选不完全，从而产生了混合叶绿体群体的杂交植株。在一些植物中，链霉素抗性和敏感性叶绿体的分选在第二代后代中仍然很明显。
Streptomycin resistance was transferred by “donor-recipient” protoplast fusion fromNicotiana tabacum(SR-1) protoplasts intoNicotiana tabacum(cytoplasmic male sterile — Line 92) protoplasts in one case and intoNicotiana sylvestrisprotoplasts in another. It is demonstrated that streptomycin resistance (SR-1) is a chloroplast marker which segregates independently from a mitochondrial marker.In the fusion experiment whereNicotiana tabacum(Line 92) was the recipient, microcalli were plated in the presence of streptomycin. In this case, chloroplast sorting out occurred at a stage preceeding plant regeneration, producing stable streptomycin resistant cybrids. In the fusion whreNicotiana sylvestriswas the recipient, no direct selection for streptomycin resistance was performed. In this case chloroplast sorting out was incomplete, thus producing cybrid plants with a mixed chloroplast population. In some plants, sorting out of streptomycin resistant and sensitive chloroplasts was still apparent in the second generation progeny.