Comparison of various basement membrane components in benign and malignant peripheral nerve tumours
Comparison of various basement membrane components in benign and malignant peripheral nerve tumours
复制标题
良恶性周围神经肿瘤各种基底膜成分的比较
作者：
S. Haraida;A. Nerlich;K. Bise;I. Wiest;E. Schleicher
采用免疫组织化学方法对周围神经组织的良、恶性肿瘤进行分析。我们检测了基底膜成分IV型胶原、层粘连蛋白、硫酸乙酰肝素蛋白多糖、纤维连接蛋白、S100蛋白以及间质III型和V型胶原的分布。层粘连蛋白普遍存在于雪旺细胞，而IV型胶原存在于神经周围细胞。当检测骨髓成分时，成纤维细胞除纤维连接蛋白外明显无反应。在良性肿瘤中观察到三种特殊的BM模式：(A)束状区(神经鞘瘤Antoni A区、丛状神经纤维瘤中心纤维束和皮肤神经纤维瘤中央区)的雪旺细胞样；(B)神经周围细胞样(神经鞘瘤的包膜结构)；(C)成纤维细胞样(粘液样和纤维转化区)。大多数恶性组织表现为不同程度的片段性层粘连蛋白灶性沉积。其他BM成分仅存在于分化较好的区域。分化较差的肿瘤仅表现为纤维连接蛋白反应。我们的结果提供了证据，BM成分的特殊染色模式有助于区分神经源性肿瘤中不同的细胞增殖。雪旺细胞不仅可以通过S100蛋白染色与周围神经细胞区分开来，还可以通过其特有的BM染色来区分。此外，神经周细胞可以从不表达BM物质的成纤维细胞中分离出来。层粘连蛋白在正常神经和良性神经肿瘤中的“趋向性”--在神经源性肉瘤中持续存在--表明这些细胞中有优先的雪旺细胞分化。
Immunohistochemical methods were used to analyse benign and malignant tumours of peripheral nerve tissue. We tested for the distribution of basement membrane (BM) components collagen IV, laminin, heparan sulphate proteoglycan, fibronectin, for S100 protein and for the presence of interstitial collagens III and V. Laminin was generally noted in association with Schwann cells, but collagen IV occurred with perineural cells. When tested for BM components, fibroblasts were notably non-reactive except for fibronectin. Three specific area-dependent BM patterns were observed in the benign tumours: (a) Schwann cell-like, in fascicular areas (Antoni A areas of schwannoma, central fibrous bundles of plexiform neurofibromas and central areas of cutaneous neurofibroma), (b) perineural cell-like (capsular structures of schwannoma) and (c) fibroblast-like (myxoid and fibrously transformed areas). Most malignant tissues showed a variably fragmentary focal deposition of laminin. Other BM components were present only in well-differentiated areas. Poorly differentiated tumours demonstrated fibronectin reactivity alone. Our results provide evidence that the specific staining pattern for BM components helps to differentiate the various cellular proliferations in neurogenic tumours. Schwann cells are not only distinguishable from perineural cells by S100 protein staining, but also by their specific BM staining. In additon, perineural cells can be separated from fibroblasts, which do not express BM material. The “tropism” of laminin in normal nerves and benign neural tumours — which persists in neurogenic sarcomas — indicates preferential Schwann cell differentiation in these cells.