Delayed Sry and Sox9 expression in developing mouse gonads underlies B6-YDOM sex reversal
Delayed Sry and Sox9 expression in developing mouse gonads underlies B6-YDOM sex reversal
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DOI：
10.1016/j.ydbio.2004.11.030
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发表时间：
2005-02-15
影响因子：
2.7
通讯作者：
Koopman, P
中科院分区：
文献类型：
--
作者：
Bullejos, M;Koopman, P
当家鼠 Y 染色体的某些变体与 C57BL/6J (136) 近交小鼠品系的遗传背景杂交时，就会出现 B6-Y-DOM 性逆转现象，该品系通常携带源自家鼠的 Y 染色体。虽然性逆转被认为涉及 Sry 结构或表达的品系特异性变异，但实际原因尚未确定。在这里，我们使用原位杂交研究了 Sry 和关键下游基因 Sox9 在含有不同染色体组合的菌株中的表达，以研究 B6-Y-DOM 性逆转的原因。我们的研究结果表明，Sry(DOM) 相对于 Sry(B6) 的表达延迟是 B6-Y-DOM 性逆转的基础，并提供了第一个分子证实，即 Sry 必须在关键时间窗口内发挥作用，以适当激活 Sox9 并在卵巢决定途径开始之前影响男性睾丸决定。 (C) 2004 Elsevier Inc. 保留所有权利。
The phenomenon of B6-Y-DOM sex reversal arises when certain variants of the Mus domesticus Y chromosome are crossed onto the genetic background of the C57BL/6J (136) inbred mouse strain, which normally carries a Mus musculus-derived Y chromosome. While the sex reversal has been assumed to involve strain-specific variations in structure or expression of Sry, the actual cause has not been identified. Here we used in situ hybridization to study expression of Sry, and the critical downstream gene Sox9, in strains containing different chromosome combinations to investigate the cause of B6-Y-DOM sex reversal. Our findings establish that a delay of expression of Sry(DOM) relative to Sry(B6) underlies B6-Y-DOM sex reversal and provide the first molecular confirmation that Sry must act during a critical time window to appropriately activate Sox9 and effect male testis determination before the onset of the ovarian-determining pathway. (C) 2004 Elsevier Inc. All rights reserved.