A FAMILY OF AMPA-SELECTIVE GLUTAMATE RECEPTORS
A FAMILY OF AMPA-SELECTIVE GLUTAMATE RECEPTORS
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DOI：
10.1126/science.2166337
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发表时间：
1990-08-03
期刊：
影响因子：
56.9
通讯作者：
SEEBURG, PH
中科院分区：
文献类型：
--
作者：
KEINANEN, K;WISDEN, W;SEEBURG, PH
从大鼠脑cDNA文库中克隆了4个编码900个氨基酸的谷氨酸受体的cDNA，它们的序列同源性约为70%。原位杂交显示整个大脑的同源mRNA的差异表达模式。cDNA在培养的哺乳动物细胞中的功能性表达产生了展示α-受体的受体。氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）-选择性结合药理学（AMPA =使君子酸盐>谷氨酸盐>红藻氨酸盐）以及由谷氨酸盐、AMPA和红藻氨酸盐门控并被6，7-二硝基喹喔啉-2，3-二酮（CNQX）阻断的阳离子通道。
Four cloned cDNAs encoding 900-amino acid putative glutamate receptors with approximately 70 percent sequence identity wree isolated from a rat brain cDNA library. In situ hybridization revealed differential expression patterns of the cognate mRNAs throughout the brain. Functional expression of the cDNAs in cultured mammalian cells generated receptors displaying .alpha.-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-selective binding pharmacology (AMPA = quisqualate > glutamate > kainate) as well as cation channels gated by glutamate, AMPA, and kainate and blocked by 6,7-dinitroquinoxaline-2,3-dione (CNQX).