Bioleaching of chalcopyrite by defined mixed moderately thermophilic consortium including a marine acidophilic halotolerant bacterium.
Bioleaching of chalcopyrite by defined mixed moderately thermophilic consortium including a marine acidophilic halotolerant bacterium.
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DOI：
10.1016/j.biortech.2012.06.114
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发表时间：
2012-10
影响因子：
11.4
通讯作者：
Yuguang Wang;Li-jun Su;Lijuan Zhang;W. Zeng;Junzi Wu;Lili Wan;G. Qiu;Xinhua Chen;Hong-bo Zhou
中科院分区：
文献类型：
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作者：
Yuguang Wang;Li-jun Su;Lijuan Zhang;W. Zeng;Junzi Wu;Lili Wan;G. Qiu;Xinhua Chen;Hong-bo Zhou
由三种陆地微生物(铁钩端螺旋体、卡氏酸性硫杆菌和嗜热铁质)和一种海洋嗜酸耐盐细菌(硫杆菌)组成的混合中度嗜热联合体。并用实时定量聚合酶链式反应(QPCR)对其群落动态进行了监测。结果表明，硫杆菌属(Sulfbacillussp.)TPY能耐受2%(w/v)的氯化钠，而当氯化钠浓度高于0.35%时，其他三种微生物受到抑制。结果表明，一定浓度的氯化钠对黄铜矿的生物浸出有一定的促进作用。通过对微生物在0.1%氯化钠溶液中浸出过程中的群落动态分析，发现硫杆菌属(Sulfbacillussp.)在整个生物浸出过程中，TPY是优势菌种，与硫杆菌相比，嗜铁乳杆菌和金黄色葡萄球菌在任何时候都较少。TPY。即使在最后阶段，嗜热镰刀菌也从来不是优势种。
A defined mixed moderately thermophilic consortium including three terrestrial microorganisms (Leptospirillum ferriphilum, Acidithiobacillus caldus and Ferroplasma thermophilum) and a marine acidophilic halotolerant bacterium (Sulfobacillus sp. TPY) was constructed to evaluate its ability for bioleaching of chalcopyrite with the addition of sodium chloride (NaCl), and the community dynamics was monitored by real-time quantitative PCR (qPCR). It was found that Sulfobacillus sp. TPY was able to tolerate 2% (w/v) NaCl, while other three microorganisms were suppressed when the concentration of NaCl was higher than 0.35%. The results suggested that NaCl below certain concentration could improve copper extraction by using pure cultures or the consortium to bioleach chalcopyrite. Community dynamics analysis during bioleaching at 0.1% NaCl showed that Sulfobacillus sp. TPY was predominant species during the whole bioleaching process, L. ferriphilum and A. caldus were less at any time compared with Sulfobacillus sp. TPY. F. thermophilum had never been dominant species even in the final stage.