Effects of an ω3 fatty acid-biased diet on luteolysis, parturition, and uterine prostanoid synthesis in pregnant mice
Effects of an ω3 fatty acid-biased diet on luteolysis, parturition, and uterine prostanoid synthesis in pregnant mice
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以 ω3 脂肪酸为主的饮食对妊娠小鼠黄体溶解、分娩和子宫前列腺素合成的影响
DOI：
10.1016/j.bbrc.2021.12.029
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发表时间：
2022
影响因子：
3.1
通讯作者：
Sugimoto Yukihiko
中科院分区：
文献类型：
--
作者：
Hashimoto Miho;Makino Nagisa;Inazumi Tomoaki;Yoshida Rina;Sugimoto Toshiko;Tsuchiya Soken;Sugimoto Yukihiko
ω3多不饱和脂肪酸（PUFAs）被认为对健康和疾病有有益的影响，因此它们的摄入是被鼓励的。然而，ω3 pufa如何影响女性生殖过程仍不清楚，其中ω6 pufa衍生的前列腺素e2和pgf2 α起着至关重要的作用。因此，我们比较了ω3 pufa偏向亚麻籽油饲粮（Lin）小鼠和ω6 pufa偏向豆油饲粮（Soy）小鼠的雌性生殖性能。林小鼠子宫内花生四烯酸（AA）和二十碳五烯酸（EPA）含量分别是大豆小鼠的0.42和16倍，EPA/AA比值为0.7（大豆小鼠为0.02）。Lin小鼠的生育指标没有变化，包括黄体溶解和分娩。Lin小鼠的子宫PG合成谱与大豆小鼠相似，但pgf2 α和pge2水平为大豆小鼠的50%，这是由于EPA/AA比的增加。大豆和林小鼠子宫组织中均未检测到pgf3 α和pge3。有趣的是，在Lin小鼠中，即使在ω6 pufa减少的情况下，“黄体溶解”的pgf2 α合成也得到了相当大的维持。这些结果表明，即使在ω - 6 pufa降低的条件下，也存在一种复杂的机制，确保pgf2 α的合成达到足以触发黄体溶解和分娩的水平。
The ω3 polyunsaturated fatty acids (PUFAs) are known to have beneficial effects on health and diseases, and hence their intake is encouraged. However, it remains unknown as to how ω3 PUFAs affect female reproduction processes, in which ω6 PUFA-derived prostaglandin (PG) E2and PGF2αplay crucial roles. We therefore compared female reproductive performance between ω3 PUFA-biased linseed oil diet-fed (Lin) mice and ω6 PUFA-biased soybean oil diet-fed (Soy) mice. In Lin mice, the uterine levels of arachidonic acid (AA) and eicosapentaenoic acid (EPA) were 0.42 fold and 16 fold of those in Soy mice, respectively, with the EPA/AA ratio being 0.7 (vs0.02 in Soy mice). Lin mice showed no alterations in any of the fertility indexes, including luteolysis and parturition. The uterine PG synthesis profiles of Lin mice were similar to those of Soy mice, but the levels of PGF2αand PGE2were 50% of those in Soy mice, as a result of the increased EPA/AA ratio. PGF3αand PGE3were undetectable in the uterine tissues of Soy and Lin mice. Interestingly, in Lin mice, ‘luteolytic’ PGF2αsynthesis was considerably maintained even in the ω6 PUFA-reduced condition. These results suggest the existence of an elaborate mechanism securing PGF2αsynthesis to a level that is sufficient for triggering luteolysis and parturition, even under ω6 PUFA-reduced conditions.