Survival and differentiation of cultured retinal progenitors transplanted in the subretinal space of the rat
Survival and differentiation of cultured retinal progenitors transplanted in the subretinal space of the rat
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DOI：
10.1006/bbrc.2000.2153
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发表时间：
2000-02-24
影响因子：
3.1
通讯作者：
Ahmad, I
中科院分区：
文献类型：
--
作者：
Chacko, DM;Rogers, JA;Ahmad, I
我们已经证明，胚胎视网膜含有祖细胞显示干细胞的特性在体外。这些细胞是增殖性的，并且除了表达神经外胚层标记物巢蛋白之外，是多能的。这些特性以及假定的干细胞在暴露于有利环境时可以分化为光感受器的事实将它们鉴定为解决退行性视网膜疾病的可行移植试剂。在这里，我们报告的生存和分化培养的视网膜祖细胞后视网膜下移植。视网膜祖细胞移植物，无论是作为神经球或游离细胞的形式，存活而不破坏宿主视网膜的形态和层状组织。他们没有形成视网膜，形态障碍重建正常解剖的视网膜。此外，移植的祖细胞表达光感受器特异性标记，表明祖细胞具有分化为光感受器的潜力。我们的观察表明，培养的视网膜祖细胞可以成为治疗性移植的可行试剂。(C)北京大学出版社.
We have shown that embryonic retina contains progenitors which display stem cell properties in vitro. These cells are proliferative and in addition to expressing the neuroectodermal marker, nestin, are multipotential. These properties and the fact that the putative stem cells can differentiate as photoreceptors when exposed to conducive environment identify them as a viable transplantation reagents to address degenerative retinal diseases. Here we report the survival and differentiation of cultured retinal progenitors upon subretinal transplantation. The retinal progenitor grafts, either as neural spheres or in the form of dissociated cells, survived without disrupting the morphology and laminar organization of the host retina. They did not form rosettes, the morphological barrier to the reconstruction of the normal anatomy of the retina. In addition, transplanted progenitors expressed photoreceptor-specific markers, suggesting that progenitors have the potential to differentiate as photoreceptors. Our observations suggest that cultured retinal progenitors can be a viable reagents for therapeutic transplantation. (C) 2000 Academic Press.