HIGH TRANSMISSION OF PATERNAL PLASTID DNA IN ALFALFA PLANTS DEMONSTRATED BY RESTRICTION FRAGMENT POLYMORPHIC ANALYSIS
HIGH TRANSMISSION OF PATERNAL PLASTID DNA IN ALFALFA PLANTS DEMONSTRATED BY RESTRICTION FRAGMENT POLYMORPHIC ANALYSIS
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DOI：
10.1007/bf00223786
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发表时间：
1990-01-01
影响因子：
5.4
通讯作者：
SORENSEN, EL
中科院分区：
文献类型：
--
作者：
MASOUD, SA;JOHNSON, LB;SORENSEN, EL
在正常绿色苜蓿植株间杂交的后代中发生了高频率的父本质粒传递。用放射性标记的苜蓿质体DNA（cpDNA）探针与后代DNA重组酶的Southern杂交分析质粒传递。每个探针揭示了一个特定的多态性区分父母质体基因组。在212个后代中，34个是异质性的，其cpDNAs的范围从主要是父系到主要是母系。从异质植株顶端生长恢复后的芽再生揭示了在给定植株中混合质体的持久性。然而，不同的芽内的绿色异质植物表现出父本，母本，或混合的cpDNAs。在一些正反交中，观察到母本核基因组对父本质体转移频率的影响。从四倍体（2n = 4.x.）= 32）苜蓿（Medicago sativa ssp.）印度大麻二倍体（2n = 2 × 104）。= 16）M.莴苣falcata杂交，由未减数配子产生。在这里，显然不仅仅是母体基因组在控制质体的传递中起作用。考虑到所有的杂交，只有5的212个后代cpDNAs缺乏一个明确的父本质体片段的证据。
A high frequency of paternal plasmid transmission occurred in progeny from crosses among normal green alfalfa plants. Plasmid transmission was anlayzed by hybridization of radiolabeled alfalfa plastid DNA (cpDNA) probes to Southern blots of restruction digests of the progeny DNA. Each probe revealed a specific polymorphism differentiating the parental plastid genomes. Of 212 progeny, 34 were heteroplastidic, with their cpDNAs ranging from predominantly paternal to predominantly maternal. Regrowth of shoots from heteroplasmic plants following revoval of top growth revealed the persistence of mixed plastids in a given plant. However, different shoots within a green heteroplasmic plant exhibited paternal, maternal, or mixed cpDNAs. Evidence of maternal nuclear genomic influence on the frequency of paternal plastid trnasmission was observed in some reciprocal crosses. A few tetraploid F1 progeny were obtained from tetraploid (2n = 4.times. = 32) Medicago sativa ssp. sativa .times. diploid (2n = 2 .times. = 16) M. sativa ssp. falcata crosses, and resulted from unreduced gametes. Here more than the maternal genome alone apparently functioned in controlling plastid trnasmission. Considering all crosses, only 5 of 212 progeny cpDNAs lacked evidence of a definitive paternal plastid fragment.