SHEAR-INDUCED ACTIVATION OF PLATELETS
SHEAR-INDUCED ACTIVATION OF PLATELETS
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DOI：
10.1016/0021-9290(79)90150-7
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发表时间：
1979-01-01
影响因子：
2.4
通讯作者：
MOCKROS, LF
中科院分区：
文献类型：
--
作者：
RAMSTACK, JM;ZUCKERMAN, L;MOCKROS, LF
富含血小板的[犬]血浆在通过试管（i.d. [内径] = 305、406和508 μ m。体积平均剪切应力为300，750和1000达因/厘米2和平均停留时间为25-1650毫秒。这些剪切这些停留时间不产生细胞溶解，但激活血小板，使他们对外源性ADP的反应降低和血小板因子3被释放。血栓弹力图测量表明剪切诱导的高凝状态。允许样品在剪切后静置30-60分钟揭示了一些指示的活化是可逆的。将抗凝剂肝素加入剪切样品中产生异常反应。的定量结果是独立的管的表面积体积比，但取决于剪切应力的水平和停留时间。
Platelet-rich [canine] plasma was subjected to shear in Poiseuille flows through tubes, i.d. [inner diameter] = 305, 406 and 508 .mu.m. Bulk-average shear stresses were 300, 750 and 1000 dynes/cm2 and the average residence times were 25-1650 ms. These shears for these residence times did not produce cell lysis, but did activate the platelets so that their response to exogenous ADP were reduced and platelet factor 3 was released. Thromboelastographic measurements indicated shear-induced hypercoagulability. Allowing samples to stand 30-60 min after being sheared revealed that some of the indicated activation was reversible. Adding the anticoagulant heparin to sheared samples produced an anomalous response. The quantitative results were independent of tube surface-volume ratio, but were dependent on the level of shear stress and on residence time.