Crystal violet-G-quadruplex complexes as fluorescent sensors for homogeneous detection of potassium ion
Crystal violet-G-quadruplex complexes as fluorescent sensors for homogeneous detection of potassium ion
复制标题
结晶紫-G-四链体复合物作为荧光传感器用于均匀检测钾离子
DOI：
10.1016/j.bios.2009.06.002
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发表时间：
2009-09-15
影响因子：
12.6
通讯作者：
Shen, Han-Xi
中科院分区：
文献类型：
--
作者：
Kong, De-Ming;Guo, Jun-Hong;Shen, Han-Xi
报道了一种新的K（+）检测方法，该方法使用无标记的G-四链体形成寡核苷酸和三苯甲烷荧光染料结晶紫（CV）。该方法是基于某些CV/G-四链体复合物在K（+）或Na（+）存在下的荧光差异，且荧光随K（+）浓度的变化而变化。根据CV的荧光变化作为离子条件的函数的性质，可以开发两种K（+）检测模式。一种是荧光减弱模式，其中使用T（3）TT（3）（5 '-GGGTTTGGGTGGGTTTGGG），CV的荧光随着K（+）浓度的增加而减弱。另一种是荧光增强模式。其中使用Hum 21（5 ′-GGGTTAGGGTTAGGGTTAGGG），CV的荧光随着K（+）浓度的增加而增加。与已发表的一些K（+）检测方法相比，该方法具有一些重要的特点。例如较低的测试成本、可耐受的较高的Na（+）浓度、可调节的线性检测范围以及较长的激发和发射波长。初步结果表明，该方法可用于生物系统，例如尿液。(C)2009爱思唯尔有限公司版权所有。
A novel K(+) detection method was reported using a label-free G-quadruplex-forming oligonucleotide and a triphenylmethane fluorescent dye crystal violet (CV). This method is based on the fluorescence difference of some CV/G-quadruplex complexes in the presence of K(+) or Na(+), and the fluorescence change with the variation of K(+) concentration. According to the nature of the fluorescence change of CV as a function of ionic conditions, two K(+) detection modes can be developed. One is a fluorescence-decreasing mode, in which T(3)TT(3) (5'-GGGTTTGGGTGGGTTTGGG) is used, and the fluorescence of CV decreases with an increased concentration of K(+). The other is a fluorescence-increasing mode. in which Hum21 (5'-GGGTTAGGGTTAGGGTTAGGG) is used, and the fluorescence of CV increases with an increased concentration of K(+). Compared with some published K(+) detection methods, this method has some important characteristics. such as lower cost of the test, higher concentrations of Na(+) that can be tolerated, adjustable linear detection range and longer excitation and emission wavelengths. Preliminary results demonstrated that the method might be used in biological systems, for example in urine. (C) 2009 Elsevier B.V. All rights reserved.