VOLATILE PRODUCTS FROM ACETYLCHOLINE AS MARKERS IN THE RAPID URINE TEST USING HEADSPACE GAS-LIQUID-CHROMATOGRAPHY
VOLATILE PRODUCTS FROM ACETYLCHOLINE AS MARKERS IN THE RAPID URINE TEST USING HEADSPACE GAS-LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/s0378-4347(00)84068-2
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
HAYWARD, NJ
中科院分区：
文献类型：
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作者：
DAVIES, TJ;HAYWARD, NJ
所有变形杆菌培养物的顶空GLC分析。结果表明，乙酰胆碱产生三甲胺可作为快速检测大肠埃希菌和克雷伯氏菌3.5h内大量奇异杆菌、普通杆菌、雷氏杆菌和不定形杆菌A的标志物。在尿液样本中。其他5种尿路病原菌培养均未检出三甲胺。在15株产气克雷伯菌中，有6株产三甲胺的菌株产乙酰胆碱，但与变形杆菌不同。通过从阿拉伯糖中生产乙醇。从乙酰胆碱出发，由奇异变形杆菌、普通变形杆菌、雷氏变形杆菌、变态变形杆菌A、变态变形杆菌B、大肠杆菌、产气性克雷伯氏菌和粪链球菌合成乙酸乙酯。
Headspace GLC analyses of cultures of all Proteus spp. showed that the production of trimethylamine from acetylcholine could be the marker for the detection in 3.5 h of significant numbers of P. mirabilis, P. vulgaris, P. rettgeri and P. inconstans A in the rapid test for Escherichia and Klebsiella spp. in urine specimens. Trimethylamine was not detected in cultures of 5 other urinary pathogens. Of 15 strains of K. aerogenes, 6 produced trimethylamine from acetylcholine but were distinguished from Proteus spp. by ethanol production from arabinose. Ethyl acetate was produced from acetylcholine by P. mirabilis, P. vulgaris, P. rettgeri, P. inconstans A, P. inconstans B, E. coli, K. aerogenes and Streptococcus faecalis.