Two Possible Barriers Blocking Conjugation Between Different Megakaryotypes of Blepharisma
Two Possible Barriers Blocking Conjugation Between Different Megakaryotypes of Blepharisma
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DOI：
10.2108/zs140151
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发表时间：
2015-01-01
影响因子：
0.9
通讯作者：
Harumoto, Terue
中科院分区：
文献类型：
--
作者：
Kobayashi, Mayumi;Miura, Mamiko;Harumoto, Terue
为了确定它们各自的配子素(交配信息素)是否能有效地诱导不同物种之间的交配对，我们研究了三种白纹伊蚊的交配对形成。波纹假单胞菌(巨核II)II型细胞无细胞培养液(CFF2)可诱导日本巴氏杆菌与巴氏杆菌I型细胞(巨核IV型)配对，日本巴氏杆菌与巴氏杆菌CFF2可诱导波氏杆菌I型细胞配对。波纹夜蛾I型细胞无细胞培养液(CFF1)不能诱导日本巴氏杆菌和波氏杆菌II型细胞配对，日本巴氏杆菌和波氏杆菌CFF1也不能诱导波纹巴氏杆菌配对。与先前报道的一样，来自B.Japan onicum和B.stoltei的CFF1相互诱导配对。这些结果表明，GAMONE 2在巨核型II和IV型中是常见的，而GAMONE 1似乎至少是巨核型特有的。当属于巨核型II和IV的细胞分别用有效配子和混合处理时，巨核型之间的交配对很少形成。综上所述，这些结果表明，至少有两个障碍，一个是伽马酮，另一个是参与配对形成的因素，阻止了不同巨核型眼睑畸形之间的结合。
We investigated mating pair formation between three Blepharisma species Blepharisma undulans, Blepharisma japonicum, and Blepharisma stoltei to determine whether their respective gamones (mating pheromones) effectively induce mating pairs between different species. Cell-free fluid from type II cells (CFF2) of B. undulans (megakaryotype II) induced pairing of B. japonicum and B. stoltei type I cells (megakaryotype IV), and CFF2 of B. japonicum and B. stoltei induced pairing of B. undulans type I cells. Cell-free fluid from B. undulans type I cells (CFF1) did not induce pairing of B. japonicum and B. stoltei type II cells, and CFF1 of B. japonicum and B. stoltei failed to induce pairing of B. undulans. CFF1 from B. japonicum and B. stoltei mutually induced pairing, as previously reported. These results indicate that gamone 2 is common among megakaryotypes II and IV, and that gamone 1 appears to be at least megakaryotype-specific. When cells belonging to megakaryotypes II and IV are separately pre-treated with effective gamones and mixed, mating pairs between megakaryotypes rarely form. Taken together, these results suggest at least two barriers, a gamone and a factor involved in pair formation, that prevent conjugation between different megakaryotypes of Blepharisma.