Replicated association of the single nucleotide polymorphism in EDG1 with marbling in three general populations of Japanese Black beef cattle.
Replicated association of the single nucleotide polymorphism in EDG1 with marbling in three general populations of Japanese Black beef cattle.
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DOI：
10.1186/1756-0500-3-66
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发表时间：
2010-03-11
影响因子：
1.8
通讯作者：
Sasaki Y
中科院分区：
文献类型：
--
作者：
Sukegawa S;Miyake T;Takahagi Y;Murakami H;Morimatsu F;Yamada T;Sasaki Y
大理石花纹是日本肉牛的一个重要经济性状，其定义为肌内脂肪的数量和分布，并以牛肉大理石花纹评分（BMS）来衡量。我们最近报道了一个单核苷酸多态性（SNP），即c.- 312 A>G，在内皮细胞分化中，鞘脂G蛋白偶联受体1（EDG 1）基因与大分县的日本黑肉牛群体中的BMS水平相关，其中G等位基因与高水平的BMS相关。因此，C. 312 A>G SNP可能是一个标记辅助选择的候选标记。在这项研究中，我们调查了这种关联是否可以在其他3个独立的日本黑牛群体中复制，并分析了SNP基因型对BMS以外的胴体性状的影响。BMS水平的统计学显着差异之间的基因型检测c.- 312 A>G SNP在宫崎县（P = 0.0377）和长崎县（P = 0.0012）的日本黑肉牛群体中存在显著差异，在鹿儿岛县群体中存在边缘差异（P = 0.0786）。SNP中的G等位基因与BMS水平的增加相关。G等位基因似乎也有一个有利的影响，如果有的话，对牛的胴体重，肋眼面积和肋骨厚度。这些发现表明，C.- 312 A>G SNP与BMS水平在日本黑肉牛群体中的重复性在其他肉牛群体中得到了复制，并揭示了G等位基因对一般日本黑肉牛群体的产肉性能的有利影响。因此，我们得出结论，C。312 A>G SNP可用于有效的标记辅助选择，以提高日本黑肉牛的BMS水平。
Marbling, defined by the amount and the distribution of intramuscular fat and measured as beef marbling score (BMS), is an economically important trait of beef cattle in Japan. We recently reported that a single nucleotide polymorphism (SNP), namely, c.-312A>G, in the endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 (EDG1) gene was associated with the BMS level in the Japanese Black beef cattle population of Oita prefecture, with the G allele being associated with a high level of the BMS. Thus, the c.-312A>G SNP seems to be a candidate marker for marker-assisted selection. In this study, we investigated whether this association could be replicated in 3 other independent Japanese Black cattle populations and analyzed the effect of the SNP genotypes on the carcass traits other than the BMS. Statistically significant differences in the BMS level were detected among the genotypes of the c.-312A>G SNP in the Japanese black beef cattle populations of Miyazaki (P = 0.0377) and Nagasaki (P = 0.0012) prefectures, and marginal difference was detected in the Kagoshima prefecture population (P = 0.0786). The G allele in the SNP was associated with an increase in the BMS level. The G allele also seemed to have a favorable influence, if any, on the carcass weight, rib eye area and rib thickness of the cattle populations. These findings suggest that the association of the c.-312A>G SNP with the BMS level in the Japanese Black beef cattle population was replicated in other beef cattle populations, and revealed favorable effects of the G allele on the beef productivity in the general Japanese Black beef cattle population. Thus, we concluded that the c.-312A>G SNP is useful for effective marker-assisted selection to increase the BMS level in Japanese Black beef cattle.