Degradation of receptor-bound human choriogonadotropin by murine Leydig tumor cells.
Degradation of receptor-bound human choriogonadotropin by murine Leydig tumor cells.
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鼠 Leydig 肿瘤细胞对受体结合的人绒毛膜促性腺激素的降解。
DOI：
10.1152/ajpendo.1979.236.2.e129
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
D. Puett
中科院分区：
文献类型：
--
作者：
M. Ascoli;D. Puett
材料和方法高度纯化的hCG（CR-119; 12，800 IU/mg）由美国国立卫生研究院（Bethesda，MD）友情提供。照相乳剂L4购自Ilford Ltd.，伊尔福德，埃塞克斯，英格兰。异甲基丁基-黄嘌呤（IBMX）得自Aldrich Chemical Co.，密尔沃基，WI. hCG的碘化。通过乳过氧化物酶法进行hCG的碘化（32）。碘化激素的比活性为30 μ l/pg。颗粒细胞培养。在发情前期的早晨，通过穿刺和温和表达从大鼠排卵前卵泡收集颗粒细胞，置于含有2 mM谷氨酰胺、100 U/ml青霉素、100 pg/ml链霉素和20%胎牛血清的麦考伊5 a（改良）培养基中。将细胞（106个细胞/ml）引入Falcon Optilux组织培养皿（35 x 10 mm）中，并按照尼姆罗德和Lindner（37）的描述培养24小时。
MATERIALS AND METHODSMateriaZs. Highly purified hCG (CR-119; 12,800 IU/mg) was kindly provided by the National Institutes of Health, Bethesda, MD. Photographic emulsion L4 was frdm Ilford Ltd., Ilford, Essex, England. Isomethylbutyl-xanthine (IBMX) was obtained from Aldrich Chemical Co., Milwaukee, WI.Iodination of hCG. Iodination of hCG was performed by the lactoperoxidase method (32). The specific activity of the iodinated hormone was 30@ i/pg. Granulosa cell culture. Granulosa cells were collected from rat preovulatory follicles on the morning of proestrus by puncture and gentle expression into McCoy’s 5a (modified) medium containing 2 mM glutamine, 100 U/ml penicillin, 100 pg/ml streptomycin, and 20% fetal calf serum. Cells (lo6 cells/ml) were introduced into Falcon Optilux tissue culture dishes (35 x 10 mm) and cultured for 24 h as described by Nimrod and Lindner (37)