Zinc oxide influences mitogen-activated protein kinase and TGF-β1 signaling pathways, and enhances intestinal barrier integrity in weaned pigs
Zinc oxide influences mitogen-activated protein kinase and TGF-β1 signaling pathways, and enhances intestinal barrier integrity in weaned pigs
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氧化锌影响丝裂原激活蛋白激酶和 TGF-β1 信号通路，并增强断奶猪肠道屏障的完整性
DOI：
10.1177/1753425914536450
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发表时间：
2015-05-01
期刊：
影响因子：
3.2
通讯作者：
Hu, Cai Hong
中科院分区：
文献类型：
--
作者：
Song, Ze He;Xiao, Kan;Hu, Cai Hong
断奶是猪一生中最重要的事件，并且总是与肠道破裂有关。虽然众所周知，氧化锌（ZnO）发挥对肠道屏障的有益作用，这些作用的机制尚未完全阐明。我们研究了ZnO是否通过丝裂原活化蛋白激酶和TGF-1信号通路保护肠屏障。选用21日龄断奶的12头公猪，随机分为2组，每组12头。结果表明，添加氧化锌可提高日增重和采食量，降低断奶后腹泻评分。ZnO改善肠道形态，如增加绒毛高度和绒毛高度：隐窝深度比，和肠道屏障功能，如增加跨上皮电阻和降低粘膜对serenum渗透4-ku FITC葡聚糖。ZnO可降低磷酸化JNK/JNK和p38/p38的比值（p-JNK/JNK和p-p38/p38），增加ERK的比值（p-ERK/ERK）。补充ZnO增加肠道TGF-1的表达。结果表明，补充ZnO激活ERK 1/2，抑制JNK和p38信号通路，并增加肠道TGF-1的表达在断奶仔猪。
Weaning is the most significant event in the life of pigs and is always related with intestinal disruption. Although it is well known that zinc oxide (ZnO) exerts beneficial effects on the intestinal barrier, the mechanisms underlying these effects have not yet been fully elucidated. We examined whether ZnO protects the intestinal barrier via mitogen-activated protein kinases and TGF-1 signaling pathways. Twelve barrows weaned at 21d of age were randomly assigned to two treatments (0 verus 2200mg Zn/kg from ZnO) for 1 wk. The results showed that supplementation with ZnO increased daily gain and feed intake, and decreased postweaning scour scores. ZnO improved intestinal morphology, as indicated by increased villus height and villus height:crypt depth ratio, and intestinal barrier function, indicated by increased transepithelial electrical resistance and decreased mucosal-to-serosal permeability to 4-ku FITC dextran. ZnO decreased the ratios of the phosphorylated to total JNK and p38 (p-JNK/JNK and p-p38/p38), while it increased the ratio of ERK (p-ERK/ERK). Supplementation with ZnO increased intestinal TGF-1 expression. The results indicate that supplementation with ZnO activates ERK 1/2, and inhibits JNK and p38 signaling pathways, and increases intestinal TGF-1 expression in weaned pigs.