PHOTOPRODUCTION OF MOLECULAR HYDROGEN BY RHODOSPIRILLUM-RUBRUM
PHOTOPRODUCTION OF MOLECULAR HYDROGEN BY RHODOSPIRILLUM-RUBRUM
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DOI：
10.1126/science.109.2840.558
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发表时间：
1949-01-01
期刊：
影响因子：
56.9
通讯作者：
KAMEN, MD
中科院分区：
文献类型：
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作者：
GEST, H;KAMEN, MD
R.在生长培养物中的红色酵母（S1株）在He、A或H2气氛中，以谷氨酸或天冬氨酸作为N源，只要存在苹果酸、富马酸或琥珀酸，就显示分子H的强烈光化学产生。在类似的条件下，如果存在苹果酸、富马酸、草酰乙酸或草酸（但不包括琥珀酸），静息细胞将产生H2。在N2或氨作为N源的气氛中，不发生H2的光产生。在甲酸盐存在下的生长产生生物体，其将在黑暗中以甲酸盐为底物和在N2气氛中放出H2，但没有观察到以甲酸盐为底物的光生H2。
R. rubrum (strain SI) in growing culture shows vigorous photochemical production of molecular H in an atmosphere of He, A, or H2 with glutamate or aspartate as source of N, provided malic, fumaric, or succinic acids are present. Under similar conditions resting cells will produce H2 provided malic, fumaric, oxaloacetic, or pyruvic (but not succinic) acid is present. In an atmosphere of N2 or with ammonia as the N source no photoproduction of H2 occurs. Growth in presence of formate produces organisms which will evolve H2 in the dark with formate as substrate and in N2 atmosphere, but no photoproduction of H2 has been observed with formate substrate.