Candida albicans cells lacking CaMCA1-encoded metacaspase show resistance to oxidative stress-induced death and change in energy metabolism
Candida albicans cells lacking CaMCA1-encoded metacaspase show resistance to oxidative stress-induced death and change in energy metabolism
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缺乏 CaMCA1 编码的元半胱天冬酶的白色念珠菌细胞表现出对氧化应激诱导的死亡和能量代谢变化的抵抗力
DOI：
10.1016/j.fgb.2008.11.001
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发表时间：
2009-02-01
影响因子：
3
通讯作者：
Jiang, YuanYing
中科院分区：
文献类型：
--
作者：
Cao, YingYing;Huang, Shan;Jiang, YuanYing
白色念珠菌是一种机会致病菌，在各种刺激下（包括氧化应激）可发生程序性细胞死亡。在这项研究中，我们发现CaMCA 1（酿酒酵母metacaspase YCA 1同源物）的缺失可以减弱氧化应激诱导的细胞死亡和caspase激活。与野生型菌株相比，Camca 1 Delta突变株在氧化胁迫条件下表现出更高的海藻糖积累和海藻糖生物合成相关基因（TPS2和TPS3）的转录水平。此外，Camca 1 Delta突变体细胞内ATP浓度和线粒体膜电位降低，内源性活性氧（ROS）产生减少。我们的研究结果表明，CaMCA 1可能通过影响能量代谢介导的氧化应激的敏感性。白色念珠菌(C)2008年爱思唯尔公司All rights reserved.
Candida albicans, an opportunistic pathogen, can undergo programmed cell death upon various stimuli, including oxidative stress. in this study, we showed that deletion of CaMCA1, a homologue of Saccharomyces cerevisiae metacaspase YCA1, could both attenuated oxidative stress-induced cell death and caspase activation. Compared to wild-type strain, Camca1 Delta mutant showed higher accumulation of trehalose and transcription of the genes related to trehalose biosynthesis (TPS2 and TPS3) under the condition of oxidative stress. Furthermore, lower intracellular ATP concentration and mitochondrial membrane potential, less endogenous reactive oxygen species (ROS) generation were observed in Camca1 Delta mutant. Our results suggest that CaMCA1 might mediate the sensitiveness to oxidative stress by affecting energy metabolism in C. albicans. (C) 2008 Elsevier Inc. All rights reserved.