Susceptibility of Clostridium perfringens to C2-C18 fatty acids
Susceptibility of Clostridium perfringens to C2-C18 fatty acids
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DOI：
10.1111/j.1472-765x.2005.01709.x
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发表时间：
2005-01-01
影响因子：
2.4
通讯作者：
Kanka, J
中科院分区：
文献类型：
--
作者：
Skrivanová, E;Marounek, M;Kanka, J
目的：测定产气荚膜梭菌菌株CCM 4435（T）和CNCTC 5459对C-2-C-18脂肪酸的敏感性，并评价pH值对葡萄糖培养物的影响。秸秆颗粒被添加到文化中，以模拟存在的固相消化道milieu.Methods和结果：脂肪酸的抗菌活性表示为一个浓度，在该浓度下，只有50%的初始葡萄糖被利用。其中月桂酸的抗菌活性最高，其次为肉豆蔻酸、癸酸、油酸和辛酸。只有菌株CNCTC 5459对亚油酸敏感。己酸和具有较短碳链的酸也不棕榈酸和硬脂酸影响底物利用率。当梭菌在秸秆颗粒存在下生长时，肉豆蔻酸、油酸和亚油酸的抗菌活性降低。在pH5.0 -5.3下用癸酸和月桂酸处理的两种菌株的培养物中，活细胞数< 10（2）ml（-1）。在pH > 6时，只有月桂酸将两种菌株的活细胞数降低至10（2）ml（-1）以下。透射电镜观察发现，月桂酸处理后细胞内外膜分离，胞浆解体。产气荚膜杆菌。其活性不受固体颗粒的影响，并且在pH > 6时不停止。研究的意义和影响：月桂酸可能是控制农场动物梭菌感染的一种手段。
Aims: To determine susceptibility of Clostridium perfringens strains CCM 4435(T) and CNCTC 5459 to C-2-C-18 fatty acids, and evaluate influence of pH in cultures grown on glucose. Straw particles were added to cultures to simulate the presence of solid phase of the digestive tract milieu.Methods and Results: Antimicrobial activity of fatty acids was expressed as a concentration at which only 50% of the initial glucose was utilized. Lauric acid showed the highest antimicrobial activity, followed by myristic, capric, oleic and caprylic acid. Only strain CNCTC 5459 was susceptible to linoleic acid. Neither caproic acid and acids with a shorter carbon chain nor palmitic and stearic acid influenced substrate utilization. The antimicrobial activity of myristic, oleic and linoleic acid decreased when clostridia were grown in the presence of straw particles. In cultures of both strains treated with capric and lauric acid at pH 5.0-5.3, the number of viable cells was < 10(2) ml(-1). Only lauric acid reduced number of viable cells of both strains below 10(2) ml(-1) at pH > 6. Transmission electron microscopy revealed separation of inner and outer membranes and cytoplasma disorganization in cells treated with lauric acid.Conclusions: Lauric acid had the highest activity towards C. perfringens among fatty acid tested. Its activity was not influenced by the presence of solid particles and did not cease at pH > 6.Significance and Impact of the Study: Lauric acid might be a means for control of clostridial infections in farm animals.