Imaging membrane interfaces and secondary protein conformation in cell cultures with vibrational infrared photothermal and phase signal (VIPPS) microscopy
Imaging membrane interfaces and secondary protein conformation in cell cultures with vibrational infrared photothermal and phase signal (VIPPS) microscopy
复制标题
使用振动红外光热和相位信号 (VIPPS) 显微镜对细胞培养物中的膜界面和二级蛋白质构象进行成像
DOI：
10.1117/12.2595736
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Sander, Michelle Y.
中科院分区：
文献类型：
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作者：
Samolis, Panagis D.;Langley, Daniel;O'Reilly, Breanna M.;Oo, Zay;Hilzenrat, Geva;Erramilli, Shyamsunder;Sgro, Allyson E.;McArthur, Sally;Sander, Michelle Y.
中红外光热显微镜在化学成像领域展示了独特的功能，包括亚衍射有限分辨率和亚细胞成像。振动红外光热和相位信号 (VIPPS) 引入了基于不同热特性的附加对比机制。这使得能够对具有重叠吸收曲线但不同热扩散特性的特征进行高对比度成像。我们的方法为在亚细胞水平上富含蛋白质的胶原基质中生长的成纤维细胞中的次级蛋白质构象的高对比度亚衍射限制成像铺平了道路。
Mid-infrared photothermal microscopy has demonstrated unique capabilities in the field of chemical imaging including sub-diffraction limited resolution and sub cellular imaging. Vibrational Infrared Photothermal and Phase Signal (VIPPS) introduces an additional contrast mechanism based on different thermal properties. This enables high contrast imaging of features with overlapping absorption profiles but different thermal diffusion characteristics. Our approach paves the way for high contrast sub-diffraction limited imaging of secondary protein conformations in fibroblast cells grown in a protein rich collagen matrix at the subcellular level.