Hydrogen sulfide treatment at the late growth stage of Saccharomyces cerevisiae extends chronological lifespan.
Hydrogen sulfide treatment at the late growth stage of Saccharomyces cerevisiae extends chronological lifespan.
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酿酒酵母生长后期的硫化氢处理可延长其实际寿命
DOI：
10.18632/aging.202738
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Liu K
中科院分区：
文献类型：
--
作者：
Shah AA;Liu B;Tang Z;Wang W;Yang W;Hu Q;Liu Y;Zhang N;Liu K
先前的研究表明，使用缓慢释放H2S供体进行终身治疗可以延长酵母的时间顺序寿命（CLS），但目前尚不清楚H2S在酵母生长过程中何时对CLS有益。在这里，我们发现在接种后96小时使用NaHS作为快速释放H2S供体的短时间H2S处理延长了酵母的CLS，而在接种后72小时之前使用NaHS处理则没有这样做。为了揭示这一机制，我们分析了接受或未接受NaHS早期和晚期处理的酵母细胞的转录组。我们发现两种治疗方法对CLS调控相关通路的影响相似。随访qPCR和ROS分析表明，早期NaHS治疗改变了一些抗氧化基因的表达，但其稳定性不足以使CLS受益。此外，转录组数据还表明，早期和晚期H2S处理对一些基因的调控不同。具体来说，我们发现YPK2（人类SGK2的同源物，也是酵母细胞壁合成的关键调节因子）的表达在NaHS处理后期显著改变，而在NaHS处理早期没有改变。最后，CLS数据揭示了YPK2在H2S调控CLS中的关键作用，表明NaHS晚期处理并未增强YPK2敲除突变体的CLS。本研究揭示了H2S诱导CLS延长的分子机制，并首次阐明了H2S处理时机对延长寿命的重要性。
Previous studies demonstrated that lifelong treatment with a slow H2S releasing donor extends yeast chronological lifespan (CLS), but it is not clear when the action of H2S benefits to CLS during yeast growth. Here, we show that short H2S treatments by using NaHS as a fast H2S releasing donor at 96 hours after inoculation extended yeast CLS while NaHS treatments earlier than 72 hours after inoculation failed to do so. To reveal the mechanism, we analyzed the transcriptome of yeast cells with or without the early and late NaHS treatments. We found that both treatments had similar effects on pathways related to CLS regulation. Follow-up qPCR and ROS analyses suggest that altered expression of some antioxidant genes by the early NaHS treatments were not stable enough to benefit CLS. Moreover, transcriptome data also indicated that some genes were regulated differently by the early and late H2S treatment. Specifically, we found that the expression of YPK2, a human SGK2 homolog and also a key regulator of the yeast cell wall synthesis, was significantly altered by the late NaHS treatment but not altered by the early NaHS treatment. Finally, the key role of YPK2 in CLS regulation by H2S is revealed by CLS data showing that the late NaHS treatment did not enhance the CLS of a ypk2 knockout mutant. This study sheds light on the molecular mechanism of CLS extension induced by H2S, and for the first time addresses the importance of H2S treatment timing for lifespan extension.
影响因子：
3.7
作者：
Kumar S;Lefevre SD;Veenhuis M;van der Klei IJ
通讯作者：
van der Klei IJ