Statistically designed enzymatic hydrolysis for optimized production of icariside II as a novel melanogenesis inhibitor.
Statistically designed enzymatic hydrolysis for optimized production of icariside II as a novel melanogenesis inhibitor.
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发表时间：
2008
影响因子：
2.8
通讯作者：
Jun-seong Park;H. Park;H. Rho;S. Ahn;Duck-Hee Kim;I. Chang
中科院分区：
文献类型：
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作者：
Jun-seong Park;H. Park;H. Rho;S. Ahn;Duck-Hee Kim;I. Chang
从淫羊藿苷水解物中分离得到淫羊藿苷I、淫羊藿苷II和淫羊藿苷三种烯酰化黄酮醇，并通过对蘑菇酪氨酸酶的抑制作用和对黑素细胞中黑色素含量的定量测定，评价了它们对黑素形成的影响。虽然这些化合物对酪氨酸酶活性没有影响，但IC50分别为10.53和11.13 MM， IC50均能有效抑制黑素含量。而与-葡萄糖苷酶和纤维素酶反应得到的icariside II，淫羊藿苷并没有完全转化为icariside II。因此，对于高纯度生产icariside II，采用响应面法优化反应，其中酶浓度为5.0 mg/ml， pH为7,37.5℃；，以8 h反应时间为中心条件，采用纤维素酶水解淫羊藿苷制备淫羊藿苷II的中心复合设计（CCD）。建立了经验模型来描述操作因素与响应（icariside II产率）之间的关系。经统计学分析，4个因素对icariside II产量均有显著影响（p<0.01）。该模型的决定系数（R2）为0.9853，最佳产糖条件为酶浓度7.5 mg/ml， pH为5,50℃，反应时间为12 h。在设计的最优条件下，预测数据与实验数据吻合较好，验证了模型的有效性。实验室中试规模也取得了成功。
Three kinds of prenylated flavonols, icariside I, icariside II, and icaritin, were isolated from an icariin hydrolysate and their effects on melanogenesis evaluated based on mushroom tyrosinase inhibition and quantifying the melanin contents in melanocytes. Although none of the compounds had an effect on tyrosinase activity, icariside II and icaritin both effectively inhibited the melanin contents with an IC50 of 10.53 and 11.13 MM, respectively. Whereas icariside II was obtained from a reaction with beta-glucosidase and cellulase, the icariin was not completely converted into icariside II. Thus, for the high-purity production of icariside II, the reaction was optimized using the response surface methodology, where an enzyme concentration of 5.0 mg/ml, pH 7, 37.5 degrees C;, and 8 h reaction time were selected as the central conditions for the central composite design (CCD) for the enzymatic hydrolysis of icariin into icariside II using cellulase. Empirical models were developed to describe the relationships between the operating factors and the response (icariside II yield). A statistical analysis indicated that all four factors had a significant effect (p<0.01) on the icariside II production. The coefficient of determination (R2) was good for the model (0.9853), and the optimum production conditions for icariside II was an enzyme concentration of 7.5 mg/ml, pH 5, 50 degrees C, and 12 h reaction time. A good agreement between the predicted and experimental data under the designed optimal conditions confirmed the usefulness of the model. A laboratory pilot scale was also successful.