Au-nanoparticles as an electrochemical sensing platform for aptamer-thrombin interaction
Au-nanoparticles as an electrochemical sensing platform for aptamer-thrombin interaction
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DOI：
10.1016/j.bios.2008.07.008
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发表时间：
2008-12-01
影响因子：
12.6
通讯作者：
Archakov, Alexander
中科院分区：
文献类型：
--
作者：
Suprun, Elena;Shurnyantseva, Victoria;Archakov, Alexander
建立了一种基于金纳米粒子传感平台和溶出伏安法的生物亲和相互作用的电化学检测新方法。极化后金表面的氧化（导致金氧化物形成）作为分析响应的基础。选择凝血酶-凝血酶结合适体对作为模型。通过抗生物素蛋白-生物素技术将适体固定在金纳米颗粒修饰的丝网印刷电极上。发现阴极峰面积与特异性吸附在电极表面的凝血酶量成正比。获得了免疫测定的典型S形校准曲线，凝血酶检测限为10（-9）M。线性范围对应于10（-8）至10（-5）M凝血酶浓度或2 x 10（-14）至2 x 10（-11）mol/电极（R = 0.996）。凝血酶与适体的结合也已经使用铁氰化物/亚铁氰化物氧化还原对作为电化学指示剂来检测。(C)2008年由Elsevier B. V.出版。
A novel electrochemical method for the detection of bioaffinity interactions based on a gold-nanoparticles sensing platform and on the usage of stripping voltammetry technique was developed. The oxidation of gold surface (resulted in gold oxide formation) upon polarization served as a basis for analytical response. As a model, thrombin-thrombin binding aptamer couple was chosen. The aptamer was immobilized on a screen-printed electrode modified with gold-nano particles by avidin-biotin technology. Cathodic peak area was found proportional to thrombin quantity specifically adsorbed onto electrode surface. Sigmoid calibration curve as is typical for immunoassay was obtained, with thrombin detection limit of 10(-9) M. Linear range corresponds from 10(-8) to 10(-5) M thrombin concentration or 2 x 10(-14) to 2 x 10(-11) mol/electrode (R = 0.996). Binding of thrombin to an aptamer has also been detected using the ferricyanide/ferrocyanide redox couple as electrochemical indicator. (C) 2008 Published by Elsevier B.V.