Cornea organoids from human induced pluripotent stem cells
Cornea organoids from human induced pluripotent stem cells
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DOI：
10.1038/srep41286
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发表时间：
2017-01-27
影响因子：
4.6
通讯作者：
Chakravarti, Shukti
中科院分区：
文献类型：
--
作者：
Foster, James W.;Wahlin, Karl;Chakravarti, Shukti
角膜是眼睛透明的最外表面，由复层上皮、胶原基质和最里面的单细胞层状内皮组成，提供眼睛2/3的屈光度。多种角膜疾病是由遗传缺陷引起的，细胞类型和细胞外基质之间的串扰会影响最终的表型。疾病的细胞培养模型可以从包括多种角膜细胞类型和细胞外基质的角膜有机物质中受益。在这里，我们介绍了人类诱导性多能性细胞衍生的有机类化合物通过连续几轮的分化程序。这些器官具有发育中的角膜的特征，含有三种不同的细胞类型，表达关键的上皮细胞、基质细胞和内皮细胞标记。角膜类器官培养为研究角膜发育过程及其在疾病条件下的破坏提供了一个强大的3D模型系统。
The cornea is the transparent outermost surface of the eye, consisting of a stratified epithelium, a collagenous stroma and an innermost single-cell layered endothelium and providing 2/3 of the refractive power of the eye. Multiple diseases of the cornea arise from genetic defects where the ultimate phenotype can be influenced by cross talk between the cell types and the extracellular matrix. Cell culture modeling of diseases can benefit from cornea organoids that include multiple corneal cell types and extracellular matrices. Here we present human iPS cell-derived organoids through sequential rounds of differentiation programs. These organoids share features of the developing cornea, harboring three distinct cell types with expression of key epithelial, stromal and endothelial cell markers. Cornea organoid cultures provide a powerful 3D model system for investigating corneal developmental processes and their disruptions in diseased conditions.