Ventral lateral geniculate input to the medial pons is necessary for visual eyeblink conditioning in rats
Ventral lateral geniculate input to the medial pons is necessary for visual eyeblink conditioning in rats
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DOI：
10.1101/lm.1572710
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发表时间：
2010-02-01
影响因子：
2
通讯作者：
Freeman, John H.
中科院分区：
文献类型：
--
作者：
Halverson, Hunter E.;Freeman, John H.
本研究调查了视觉延迟眨眼调节所必需的条件刺激（CS）途径。最初对大鼠进行眨眼调节，刺激外侧膝状体 (LGNv) 的腹侧核作为 CS，然后在单独的训练阶段用光和音调 CS 进行调节。每个训练阶段后将蝇蕈醇注入脑桥内侧核 (MPN)，以检查每个 CS 的条件反应 (CR) 保留情况。用荧光蝇蕈醇检查了针对 MPN 的蝇蕈醇输注的扩散。 MPN 中输注蕈醇会导致 LGNv 刺激和轻度 CS 的 CR 保留严重受损。音调 CS 观察到的损伤不太严重。结果表明，来自 LGNv 和轻 CS 的 CS 信息通过 MPN 传递到小脑。荧光金 (FG) 逆行追踪显示 LGNv 和视束核对 MPN 有同侧投影。从 LGNv 和视束核到 MPN 的单侧输入可能是视觉 CS 通路的一部分，这是视觉眨眼调节所必需的。
The conditioned stimulus (CS) pathway that is necessary for visual delay eyeblink conditioning was investigated in the current study. Rats were initially given eyeblink conditioning with stimulation of the ventral nucleus of the lateral geniculate (LGNv) as the CS followed by conditioning with light and tone CSs in separate training phases. Muscimol was infused into the medial pontine nuclei (MPN) after each training phase to examine conditioned response (CR) retention to each CS. The spread of muscimol infusions targeting the MPN was examined with fluorescent muscimol. Muscimol infusions into the MPN resulted in a severe impairment in retention of CRs with the LGNv stimulation and light CSs. A less severe impairment was observed with the tone CS. The results suggest that CS information from the LGNv and light CSs is relayed to the cerebellum through the MPN. Retrograde tracing with fluoro-gold (FG) showed that the LGNv and nucleus of the optic tract have ipsilateral projections to the MPN. Unilateral inputs to the MPN from the LGNv and nucleus of the optic tract may be part of the visual CS pathway that is necessary for visual eyeblink conditioning.