Interference of Salmonella enteritidis and Lactobacillus spp. with IL-8 levels and transepithelial electrical resistance of enterocyte-like Caco-2 cells
Interference of Salmonella enteritidis and Lactobacillus spp. with IL-8 levels and transepithelial electrical resistance of enterocyte-like Caco-2 cells
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DOI：
10.1007/bf02931810
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发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Marinsek-Logar, R.
中科院分区：
文献类型：
--
作者：
Fajdiga, S.;Koninkx, J. F. J. G.;Marinsek-Logar, R.
Caco-2细胞（表现出成熟绒毛肠细胞的特征）用于确定细菌（引起人类胃肠炎的沙门氏菌）-肠细胞相互作用。研究了细菌对过滤培养的Caco-2细胞的跨上皮电阻（TEER）的干扰以及细胞暴露于S.芽孢杆菌857和/或乳杆菌属菌株（L. gasseri LF 221和L. rhamnosus BGT 10）进行评价。菌株857降低了过滤生长的Caco-2细胞的TEER;相反，乳酸杆菌几乎没有影响。S.如果Caco-2细胞与乳酸杆菌预孵育，则TEER上的乳酸杆菌浓度降低。该菌株诱导高水平的IL-8（其可导致细胞损伤）。与S. Caco-2细胞同时暴露于S.乳酸杆菌和乳酸杆菌在短暂恢复期后抑制IL-8的合成。
Caco-2 cells (exhibiting characteristics of mature villus enterocytes) were used to determine bacteria (Salmonella enteritidis causing human gastroenteritis)-intestinal cell interactions. The interference of bacteria with the transepithelial electrical resistance (TEER) of filter-grown Caco-2 cells and the production of IL-8 after exposure of the cells to S. enteritidis 857 and/or Lactobacillus strains (L. gasseri LF221 and L. rhamnosus BGT10) was evaluated. The strain 857 decreased TEER of filter-grown Caco-2 cells; in contrast, lactobacilli had a little or no effect. The effect of S. enteritidis, on the TEER decreased if Caco-2 cells were pre-incubated with lactobacilli. This strain induced high levels of IL-8 (which can lead to cell damage). Compared to the IL-8 synthesis after exposure of Caco-2 cells to S. enteritidis 857, simultaneous exposure of Caco-2 cells to S. enteritidis and lactobacilli inhibited the IL-8 synthesis after short recovery periods.