Effects of light on development of mammalian zygotes
Effects of light on development of mammalian zygotes
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DOI：
10.1073/pnas.0706687104
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发表时间：
2007-09-04
影响因子：
11.1
通讯作者：
Yanagimachi, Ryuzo
中科院分区：
文献类型：
--
作者：
Takenaka, Manami;Horiuchi, Toshitaka;Yanagimachi, Ryuzo
一般认为光对卵母细胞、受精卵或早期胚胎的生理没有影响。因此，在体外处理这些细胞的过程中，很少或根本没有注意光照条件。在这里，我们表明，冷白色荧光灯，丰富的短波长可见光和常用的研究和临床实验室，产生更多的活性氧比温暖的白色荧光灯在小鼠和仓鼠受精卵。小鼠胚泡，从受精卵遮光最好的发展到足月胎儿，其次是那些暴露于温暖的白色荧光灯，然后是那些暴露于冷白色荧光灯。我们推测光是影响胚胎环境的物理因素之一，它对哺乳动物受精卵和胚胎的影响不容忽视。
It is generally assumed that light has no effect on the physiology of oocytes, zygotes, or early embryos. Therefore, little or no attention has been paid to lighting conditions during the handling of these cells in vitro. Here we show that cool white fluorescent light, rich in short-wavelength visible light and commonly used in research and clinical laboratories, produces more reactive oxygen species in mouse and hamster zygotes than does warm white fluorescent light. Mouse blastocysts that developed from zygotes shielded from light best developed to term fetuses followed by those exposed to warm white fluorescent light and then by those exposed to cool white fluorescent light. We hypothesized that light is one of the physical factors affecting embryonic environment and that its effects on cultured mammalian zygotes and embryos should not be overlooked.