Regulation of bifurcated cytokine induction by surface charge of nanoparticles during interaction between CpG oligodeoxynucleotides and toll-like receptor 9
Regulation of bifurcated cytokine induction by surface charge of nanoparticles during interaction between CpG oligodeoxynucleotides and toll-like receptor 9
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DOI：
10.1016/j.jddst.2015.08.008
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发表时间：
2015-10-01
影响因子：
5
通讯作者：
Hanagata, Nobutaka
中科院分区：
文献类型：
--
作者：
Chinnathambi, Shanmugavel;Pi, Xiaodong;Hanagata, Nobutaka
Toll样受体9识别CpG寡脱氧核苷酸（ODNs）并诱导免疫介导细胞因子。天然磷酸二酯类B CpG ODN诱导白细胞介素-6（IL-6），但不诱导干扰素-α（IFN-α）。我们制备了具有不同正表面电荷密度的硅纳米颗粒（Si NPs），并通过静电作用与带负电荷的B类CpG ODN分子结合。未观察到B类CpG ODN分子的量或分子与裸NP结合后的负表面电荷的显著差异。然而，从外周血单个核细胞的细胞因子诱导的配置文件相关的正表面电荷密度的裸纳米颗粒结合之前的CpG ODN分子。随着正表面电荷密度的增加，IL-6诱导的水平略微降低，而IFN-α诱导随着正表面电荷密度的增加而显著增加。该观察结果表明，分叉的细胞因子诱导可以通过裸NP的表面电荷来调节。(C)2015 Elsevier B. V.版权所有。
Toll-like receptor 9 recognizes CpG oligodeoxynucleotides (ODNs) and induces immune-mediator cytokines. Natural phosphodiester class B CpG ODNs induce interleukin-6 (IL-6), but not interferon-alpha (IFN-alpha). We prepared silicon nanoparticles (Si NPs) with different positive surface charge density and bound negatively charged class B CpG ODN molecules electrostatically. No significant differences in the amount of class B CpG ODN molecules or negative surface charge after binding of the molecules onto naked NPs was observed. However, the profile of cytokine induction from peripheral blood mononuclear cells was correlated with a positive surface charge density of naked NPs prior to binding of CpG ODN molecules. The level of IL-6 induction slightly decreased as the positive surface charge density was increased, while the IFN-alpha induction significantly increased as the positive surface charge density was increased. This observation demonstrates that the bifurcated cytokine induction can be regulated by the surface charge of naked NPs. (C) 2015 Elsevier B.V. All rights reserved.