STUDIES ON THE SELECTIVITY OF DNA PRECIPITATION BY SPERMINE
STUDIES ON THE SELECTIVITY OF DNA PRECIPITATION BY SPERMINE
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DOI：
10.1093/nar/9.20.5493
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
MCCLURE, WR
中科院分区：
文献类型：
--
作者：
HOOPES, BC;MCCLURE, WR
我们研究了精胺沉淀DNA的选择性。我们已经发现，精胺诱导的DNA分子内和分子间缩合是高度选择性的，即使在添加蛋白质或三磷酸盐的存在下。我们还研究了缓冲液组分对DNA沉淀所需的精胺阈值浓度的影响。还描述了利用沉淀反应的选择性的代表性应用。
We have examined the selectivity of the precipitation of DNA by spermine. We have found that the intra- and intermolecular condensation of DNA induced by spermine is highly selective even in the presence of added protein or triphosphates. We have also investigated the influence of buffer components on the threshold concentration of spermine required for DNA precipitation. Representative applications exploiting the selectivity of the precipitation reaction are also described.