EARLY REGION-4 SEQUENCE AND BIOLOGICAL COMPARISON OF 2 ISOLATES OF MOUSE ADENOVIRUS TYPE-1
EARLY REGION-4 SEQUENCE AND BIOLOGICAL COMPARISON OF 2 ISOLATES OF MOUSE ADENOVIRUS TYPE-1
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DOI：
10.1016/0042-6822(91)90030-f
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发表时间：
1991-01-01
期刊：
影响因子：
3.7
通讯作者：
SPINDLER, KR
中科院分区：
文献类型：
--
作者：
BALL, AO;BEARD, CW;SPINDLER, KR
测定了小鼠1型腺病毒（MAV-1）88-100图单位的DNA序列。一个翻译开放阅读框显示与人腺病毒2型早期区4蛋白48%的序列相似性。基于蛋白质相似性、基因组位置和转录极性，我们得出结论，MAV-1的该区域对应于早期区域4。在该区域发现了一个由10个不完全重复序列组成的241 bp的序列，与小卫星DNA的序列相似。两个不同的传代历史的病毒分离株进行了检查，并发现在这一区域内的序列多态性。比较了两种病毒在细胞培养物和小鼠中的生长情况，仅在体内观察到微小的数量差异。
The DNA sequence of 88–100 map units of mouse adenovirus type 1 (MAV-1) was determined. One translational open reading frame showed 48% sequence similarity to a human adenovirus type 2 early region 4 protein. Based on the protein similarity, genome location, and transcriptional polarity, we concluded that this region of MAV-1 corresponds to early region 4. A 241-bp sequence consisting of 10 imperfect direct repeats with sequence similarity to minisatellite DNA was found in this region. Two virus isolates with different passage histories were examined and were found to have a sequence polymorphism within this region. The two viruses were compared for growth in cell culture and mice and small quantitative differences were observed onlyin vivo.