Roles of the Nanog protein in murine F9 embryonal carcinoma cells and their endoderm-differentiated counterparts
Roles of the Nanog protein in murine F9 embryonal carcinoma cells and their endoderm-differentiated counterparts
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DOI：
10.1038/sj.cr.7310067
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发表时间：
2006-07-01
期刊：
影响因子：
44.1
通讯作者：
Yao, Zhen
中科院分区：
文献类型：
--
作者：
Chen, Yanmei;Du, Zhongwei;Yao, Zhen
Nanog是最近发现的一种同源域转录因子，其维持胚胎干细胞（ES）的多能性并阻止其分化成内胚层。鼠F9胚胎癌细胞系是内胚层细胞谱系分化的有据可查的模型系统。在这里，我们研究了Nanog在F9细胞内胚层分化中的功能。Nanog的过表达使F9细胞恢复到ES细胞的早期状态，并抑制F9细胞中原始内胚层和壁层内胚层的分化，而对内脏内胚层的分化没有影响。相反，C-末端结构域截短的Nanog的表达自发地促进F9细胞中的内胚层分化。这些数据表明Nanog是维持F9细胞适当的未分化状态所必需的，并且Nanog的C-末端结构域在抑制F9细胞中的原始内胚层和壁内胚层分化中转导最多的作用。
Nanog is a recently discovered homeodomain transcription factor that sustains the pluripotency of embryonic stem (ES) cells and blocks their differentiation into endoderm. The murine F9 embryonal carcinoma cell line is a well-documented model system for endoderm cell lineage differentiation. Here, we examined the function of Nanog in F9 cell endoderm differentiation. Over-expression of Nanog returns the F9 cells to the early status of ES cells and represses the differentiation of primitive endoderm and parietal endoderm in F9 cells, whereas it has no effect on the differentiation of visceral endoderm. In contrast, the expression of C-terminal domain-truncated Nanog spontaneously promotes endoderm differentiation in F9 cells. These data suggest that Nanog is required to sustain the proper undifferentiated status of F9 cells, and the C-terminal domain of Nanog transduces the most effects in repressing primitive endoderm and parietal endoderm differentiation in F9 cells.