A minimally invasive and reversible system for chronic recordings from multiple brain sites in macaque monkeys
A minimally invasive and reversible system for chronic recordings from multiple brain sites in macaque monkeys
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DOI：
10.1016/j.jneumeth.2009.04.024
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发表时间：
2009-07-30
影响因子：
3
通讯作者：
Vidyasagar, T. R.
中科院分区：
文献类型：
--
作者：
Pigarev, I. N.;Saalmann, Y. B.;Vidyasagar, T. R.
我们已经开发了一种可逆系统，可以同时对经过训练的猕猴的多个大脑区域进行记录。它由一个近乎圆形的光环组成，在猴子的头部周围安装了5-10根薄塑料或不锈钢柱子，分别突出在头骨上或拧入头骨中。这两种植入柱子的方法都很容易逆转，可以进行多年的长时间录音，并训练猴子完成更复杂的任务。前者更适用于间隔较长的较短时间段(2-4个月)的记录，而后者用于一次较长时间段(6-12个月)的记录。使用这两个系统，基本上整个头皮都是完好无损的，允许同时从许多背侧皮质区域以及其他区域进行多点记录。这些记录是通过直径通常不到2毫米的微小开颅手术进行的，这些开颅手术配备了作为微电极导向管的小塑料锥体。与传统方法相比，这些程序涉及的手术相对较小，植入物通常也没有感染，因此几乎不需要维护记录室。(C)2009爱思唯尔B.V.保留所有权利。
We have developed a reversible system for performing simultaneous recordings from multiple brain areas of trained macaque monkeys. It consists of a near-circular halo fitted around the head of the monkey with 5-10 thin plastic or stainless steel posts that either jut against or are screwed into the skull, respectively. Both methods of implantation of the posts are easily reversible, enabling protracted recordings over many years and training the monkeys in more complex tasks. The former is more useful for shorter periods of recordings (2-4 months) separated by long intervals and the latter for longer periods of recordings at a time (6-12 months). With both systems, essentially the entire scalp is intact, allowing multi-site recordings from a number of dorsal cortical areas, as well as other areas, simultaneously. These recordings are performed through tiny craniotomies of usually less than 2 mm diameter, which are fitted with small plastic cones that serve as guide tubes for the microelectrodes. The surgery involved in these procedures is relatively minor compared to classical methods and the implants are also usually free of infections, thus requiring little maintenance of recording chambers. (C) 2009 Elsevier B.V. All rights reserved.