Observation of inflammatory responses in mice orally fed with bacteriophage T7
Observation of inflammatory responses in mice orally fed with bacteriophage T7
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DOI：
10.1111/jam.12565
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发表时间：
2014-09-01
影响因子：
4
通讯作者：
Myung, H.
中科院分区：
文献类型：
--
作者：
Park, K.;Cha, K. E.;Myung, H.
目的：分析小鼠摄入噬菌体后的炎症免疫反应。方法和结果：噬菌体治疗正在成为一种有效的替代常规抗生素。然而，它在人类和动物身上的安全性仍然是一个主要问题。本研究研究了口服噬菌体对小鼠炎症反应的影响。小鼠分别用小鼠诺如病毒（MNV）或噬菌体T7喂养10天，然后处死。两组均未观察到与饮食、运动或排便有关的行为变化。炎症细胞因子分析显示，MNV喂养小鼠血清中白细胞介素1 α (IL-1 α)、1 β (IL-1 β)、2 （IL-2）、10 （IL-10）、12 （IL-12）、17A （IL-17A）、干扰素- γ (ifn - α)、肿瘤坏死因子- α (tnf - α)、粒细胞集落刺激因子（G-CSF）和粒细胞巨噬细胞集落刺激因子（GM-CSF）水平升高，而噬菌体T7喂养小鼠血清中IL-17A水平仅略有升高。对胃、小肠和结肠组织样本的组织病理学分析未发现明显的病理改变。结论：噬菌体饮食只引起小鼠轻微的炎症反应。研究的意义和影响：本研究对口服噬菌体治疗的安全性问题提供了见解。
Aims: Analysis of inflammatory immune response upon bacteriophage intake in mice.Methods and Results: Bacteriophage therapy is emerging as an effective alternative to conventional antibiotics. However, its safety when applied to humans and animals remains a prime concern. This study investigated the inflammatory responses in mice fed orally with bacteriophages. The mice were fed with either murine norovirus (MNV) or bacteriophage T7 for 10 days and then sacrificed. No behavioural changes related to diet, movement or defecation were observed in either group. The inflammatory cytokine profiling showed an increased level of interleukins 1 alpha (IL-1 alpha), 1 beta (IL-1 beta), 2 (IL-2), 10 (IL-10), 12 (IL-12), 17A (IL-17A), interferon-gamma (IFN-alpha), tumour necrosis factor-alpha (TNF-alpha), granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF) in the serum from the mice fed with MNV, whereas only a slight increase in IL-17A was observed in the serum from the mice fed with bacteriophage T7. A histopathological analysis of tissue samples from the stomach, small intestine and colon revealed no significant pathological change.Conclusions: The bacteriophage diet only caused a minimal inflammatory response in the mice.Significance and Impact of the Study: This study provides insights into safety concerns of orally fed bacteriophage therapy.