ANALYSIS OF REPERTOIRE OF ANTI-NP ANTIBODIES IN C57BL-6 MICE BY CELL-FUSION .1. CHARACTERIZATION OF ANTIBODY FAMILIES IN PRIMARY AND HYPER-IMMUNE RESPONSE
ANALYSIS OF REPERTOIRE OF ANTI-NP ANTIBODIES IN C57BL-6 MICE BY CELL-FUSION .1. CHARACTERIZATION OF ANTIBODY FAMILIES IN PRIMARY AND HYPER-IMMUNE RESPONSE
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DOI：
10.1002/eji.1830080605
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发表时间：
1978-01-01
影响因子：
5.4
通讯作者：
RAJEWSKY, K
中科院分区：
文献类型：
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作者：
RETH, M;HAMMERLING, GJ;RAJEWSKY, K
将来自对半抗原（4-羟基-3-硝基苯基）乙酰基NP敏感的C57BL/6小鼠的脾细胞与骨髓瘤细胞杂交，分离出多种分泌均质抗NP抗体的杂交细胞系。通过亲和层析纯化抗体并测定其链组成、亲和力和精细特异性。从初次免疫反应中恢复的所有抗体都携带λ。光和.mu。或γ1 H链。它们的可变部分不相同，但在半抗原结合特异性方面相似，对交叉反应半抗原（4-羟基-3,5-二硝基-苯基）乙酰基（NNP）和（4-羟基-5-碘-3-硝基-苯基）乙酰基（NIP）的亲和力比同源半抗原NP更高。这种异晶特性以及 lambda.、.mu 的存在。和γ1链是C57BL/6小鼠的初级抗NP血清的特征。四个抗 NP 抗体家族，每个家族都具有特征性的精细特异性模式，属于源自超免疫抗 NP 反应的杂交细胞抗体。这些家族中的一个的抗体与从初次免疫反应中恢复的抗体相关，因为它们是异粘附的并且携带λ。轻链。其他组的所有成员都表达了.kappa。链并且是非异斜的。在该系统中发现明确的抗体家族并分离其成员使我们能够以新的方式解决 V 基因表达和多样化的问题。
Spleen cells from C57BL/6 mice sensitized to the hapten (4-hydroxy-3-nitrophenyl)acetyl NP) were hybridized with myeloma cells, and a variety of hybrid cell lines were isolated which secreted homogeneous anti-NP antibodies. The antibodies were purified by affinity chromatography and their chain composition, affinity and fine specificity were determined. All antibodies recovered from the primary immune response carried .lambda. light and .mu. or .gamma.1 H-chains. Their variable portions were nonidentical but similar in terms of hapten-binding specificity with a higher affinity for the cross-reacting haptens (4-hydroxy-3,5-dinitro-phenyl)acetyl (NNP) and (4-hydroxy-5-iodo-3-nitro-phenyl)acetyl (NIP) than for the homologous hapten NP. This heteroclitic property as well as the presence of .lambda., .mu. and .gamma.1 chains are characteristic for primary anti-NP sera of C57BL/6 mice. Four families of anti-NP antibodies, each with a characteristic fine specificity pattern, were among the hybrid cell antibodies derived from the hyperimmune anti-NP response. The antibodies of 1 of these families were related to the antibodies recovered from the primary immune response in that they were heteroclitic and carried .lambda. light chains. All members of the other groups expressed .kappa. chains and were nonheteroclitic. The finding of well-defined antibody families in this system and the isolation of their members enable us to approach the problem of V gene expression and diversification in a new way.