Blood Meal Identification and Prevalence of Avian Malaria Parasite in Mosquitoes Collected at Kushiro Wetland, a Subarctic Zone of Japan
Blood Meal Identification and Prevalence of Avian Malaria Parasite in Mosquitoes Collected at Kushiro Wetland, a Subarctic Zone of Japan
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DOI：
10.1603/me11053
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发表时间：
2011-07-01
影响因子：
2.1
通讯作者：
Yukawa, Masayoshi
中科院分区：
文献类型：
--
作者：
Ejiri, Hiroko;Sato, Yukita;Yukawa, Masayoshi
在日本，已对温带和亚热带地区鸟类和蚊子中的禽疟原虫流行情况进行了部分调查；然而，日本亚北极地区的蚊子尚未得到充分调查。本研究在日本亚北极地区钏路湿地采集蚊虫，并对蚊虫样本进行禽疟原虫检测，以证实禽疟原虫在媒介蚊与鸟类之间的传播。2008年、2009年和2010年夏季共采集18种无食蚊5657只和8种血食蚊320只。经聚合酶链反应检测，3只以北海道梅花鹿为食的伊蚊和1只未以北海道梅花鹿为食的淡色库蚊均呈禽疟原虫阳性。这是首次从分布在日本亚北极地区的蚊子中检测到禽疟原虫DNA。在圈养动物和野生动物中，成功鉴定出了7种哺乳动物、4种鸟类和1种两栖动物的血食。结果表明，日本北海道钏路湿地地区存在禽疟原虫感染禽，且禽与蚊子有直接接触。
In Japan, the prevalence of avian Plasmodium in birds and mosquitoes has been partially examined in the temperate and subtropical zones; however, mosquitoes in the Japanese subarctic zone have not been adequately investigated. In this study, mosquito collections and avian Plasmodium detections from the mosquito samples were carried out to demonstrate the avian Plasmodium transmission between vector mosquitoes and birds inhabiting in Kushiro Wetland, subarctic zone of Japan. A total of 5,657 unfed mosquitoes from 18 species and 320 blood-fed mosquitoes from eight species was collected in summer 2008, 2009, and 2010. Three Aedes esoensis that fed on Hokkaido Sika Deer and one unfed Culex pipiens group were found to be positive for avian Plasmodium by polymerase chain reaction. This is the first report of the detection of avian Plasmodium DNA from mosquitoes distributing in the subarctic zone of Japan. The blood meals were successfully identified to captive or wild animals, including seven mammalian species, four bird species, and one amphibian species. These results indicated that infected birds with avian Plasmodium inhabited and direct contacts occurred between the infected birds and mosquitoes in Kushiro Wetland, Hokkaido, Japan.