Recovery of elasticity of aged human epithelial cells in vitro
Recovery of elasticity of aged human epithelial cells in vitro
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DOI：
10.1016/j.nano.2005.12.002
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发表时间：
2006-03-01
影响因子：
5.4
通讯作者：
Woodworth, Craig D.
中科院分区：
文献类型：
--
作者：
Sokolov, Igor;Iyer, Swaminathan;Woodworth, Craig D.
我们最近发现，在体外老化过程中，人类上皮细胞的刚度有相当大的增加。这一点很重要，因为随着年龄的增长，上皮组织的弹性丧失会导致许多人类疾病。我们还发现，培养的细胞有三个不同的刚性区域，刚性的增加与细胞骨架纤维密度的增加相关。然而，不清楚哪种类型的纤维是重要的。在这项研究中，原子力显微镜和免疫荧光显微镜的特点，在体外老化的人上皮细胞，细胞松弛素B治疗前后。我们发现，与刚度增加相关的纤维主要是F-肌动蛋白微丝。此外，使用细胞松弛素13，一种抑制F-肌动蛋白聚合的化学物质，我们同时在所有三个刚性区域将老年细胞的刚性恢复到年轻水平。这些结果阐明了细胞力学在体外老化过程中的变化，它们可能与治疗上皮组织中与年龄相关的弹性丧失有关。(c)2006年爱思唯尔公司All rights reserved.
We recently found a considerable increase in rigidity of human epithelial cells during aging in vitro. This is important because the loss in elasticity of epithelial tissues with aging contributes to many human diseases. We also found that cultured cells had three distinct regions of rigidity and that the increase in rigidity correlated with an increase in density of cytoskeletal fibers. However, it was not clear which type of fiber was important. Atomic force microscopy and immunofluorescence microscopy were used in this study to characterize aging human epithelial cells in vitro, both before and after treatment with cytochalasin B. We found that the fibers associated with increased rigidity were mostly F-actin microfilaments. Furthermore, using cytochalasin 13, a chemical that inhibits polymerization of F-actin, we restored the rigidity of old cells to the young level in all three areas of rigidity simultaneously. These results clarify how the cell mechanics changes during aging in vitro, and they may be relevant for treatment of age-related loss of elasticity in epithelial tissues. (c) 2006 Elsevier Inc. All rights reserved.