Ultraviolet light-induced oxidative stress: Effects on antioxidant response of Helicoverpa armigera adults
Ultraviolet light-induced oxidative stress: Effects on antioxidant response of Helicoverpa armigera adults
复制标题
紫外线诱导的氧化应激：对棉铃虫成虫抗氧化反应的影响
DOI：
10.1016/j.jinsphys.2009.03.003
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发表时间：
2009-06-01
影响因子：
2.2
通讯作者：
Lei, Chao-Liang
中科院分区：
文献类型：
--
作者：
Meng, Jian-Yu;Zhang, Chang-Yu;Lei, Chao-Liang
紫外线（UV）灯（黑光灯），其发射在320-400 nm范围内的UV，已经在世界范围内用于昆虫害虫的光诱捕。在这篇文章中，我们测试的假设，紫外线照射的影响之一是增加对昆虫的氧化应激。研究了紫外线对棉铃虫成虫总抗氧化能力、丙二醛（MDA）和蛋白质羰基含量以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POX）和谷胱甘肽-S-转移酶（GST）活性的影响。成虫暴露于UV光不同的时间段（0，30，60和90分钟）。结果表明，紫外线照射30 min，可显著提高玉米叶片的总抗氧化能力、蛋白质羰基含量以及SOD、CAT、POX和GST活性。暴露60和90 min后，蛋白质羰基含量、CAT和GST活性均显著高于对照。然而，抗氧化能力和SOD活性恢复到对照水平，和POX活性下降，在60和90分钟。我们的研究结果证实了假设，紫外线照射增加的氧化应激水平在H。棉铃虫成虫(C)2009爱思唯尔有限公司版权所有。
Ultraviolet (UV) light (blacklight), which emits UV in the range of 320-400 nm, has been used worldwide in light trapping of insect pests. In this article, we test the hypothesis that one of the effects of UV light irradiation is to increase oxidative stress on insects. The effects of UV light irradiation on total antioxidant capacity, malondialdehyde (MDA) and protein carbonyl contents and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidases (POX) and glutathione-S-transferase (GST) were investigated in Helicoverpa armigera adults. The adults were exposed to UV light for various time periods (0, 30, 60 and 90 min). We found that exposure to UV light for 30 min resulted in increased total antioxidant capacity, protein carbonyl content and activities of SOD, CAT, POX and GST. When the exposure time lasted for 60 and 90 min, the protein carbonyl content and activities of CAT and GST remained significantly higher than the control. However, the antioxidant capacity and SOD activity returned to control levels, and POX activity decreased at 60 and 90 min. Our results confirm the hypothesis that UV light irradiation increases the level of oxidative stress in H. armigera adults. (C) 2009 Elsevier Ltd. All rights reserved.