Reduction of allergic airway eosinophilia by dietary raffinose in Brown Norway rats
Reduction of allergic airway eosinophilia by dietary raffinose in Brown Norway rats
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DOI：
10.1079/bjn20041179
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发表时间：
2004-08-01
影响因子：
3.6
通讯作者：
Kasai, T
中科院分区：
文献类型：
--
作者：
Watanabe, H;Sonoyama, K;Kasai, T
据报道，口服棉子糖，一种自然产生的不可消化的低聚糖，已经改善了人类受试者的特应性皮炎，尽管机制尚不清楚。作为特应性疾病模型，本研究探讨了膳食棉子糖对卵清蛋白致敏的棕色挪威大鼠变应原诱导的呼吸道嗜酸性粒细胞增多症的影响。棕色挪威大鼠于第0天皮下注射卵白蛋白免疫，饲喂对照饲料或添加棉子糖的饲料(50g/kg饲料)。第20天雾化吸入卵清蛋白，次日取肺泡灌洗液。喂食棉子糖饮食的大鼠的体液中嗜酸性粒细胞数量明显低于喂食对照饮食的大鼠。膳食棉子糖显著降低肺组织中IL-4和IL-5mRNA水平，并有降低卵清蛋白特异性Ig E水平的趋势。在盲肠切除和给予新霉素的大鼠中，仍然观察到饮食中棉子糖对嗜酸性粒细胞增多的抑制作用，这表明结肠菌对棉子糖的作用几乎没有贡献。腹腔注射棉子糖也可抑制嗜酸性粒细胞增多。灌胃给药后，门静脉和腹主动脉血浆中检测到显著浓度的棉籽糖。总体而言，研究结果表明，饮食中的棉子糖至少部分地通过吸收后机制改善了布朗·挪威大鼠的过敏性呼吸道嗜酸性粒细胞增多症。
Oral administration of raffinose, a naturally occurring indigestible oligosaccharide, has reportedly ameliorated atopic dermatitis in human subjects although the mechanism is unknown. The present study investigated the effect of dietary raffinose on allergen-induced airway eosinophilia in ovalbumin-sensitised Brown Norway rats as an atopic disease model. Brown Norway rats were immunised by subcutaneous injection with ovalbumin on day 0 and fed either a control diet or the diet supplemented with raffinose (50 g/kg diet). The rats were exposed to aerosolised ovalbumin on day 20, and broncho-alveolar lavage fluid was obtained on the next day. The number of eosinophils in the fluid was significantly lower in the rats fed the raffinose diet than in those fed the control diet. Dietary raffinose significantly reduced IL-4 and IL-5 mRNA levels in lung tissue and tended to lower ovalbumin-specific Ig E levels. Suppression of eosinophilia by dietary raffinose was still observed in caecectomised and neomycin-administered rats, suggesting little contribution by the colonic bacteria to the effect of raffinose. Intraperitoneal administration of raffinose also suppressed eosinophilia. Significant concentrations of raffinose were detected in portal venous and abdominal arterial plasma after the intragastric administration of raffinose. Overall, the findings suggest that dietary raffinose ameliorates allergic airway eosinophilia at least partly via post-absorptive mechanisms in Brown Norway rats.