Biological surface engineering: a simple system for cell pattern formation.
Biological surface engineering: a simple system for cell pattern formation.
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DOI：
10.1016/s0142-9612(99)00014-9
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发表时间：
1999-07
期刊：
影响因子：
14
通讯作者：
Shuguang Zhang;Lin Yan;M. Altman;M. Lässle;H. Nugent;F. Frankel;D. Lauffenburger;G. Whitesides
中科院分区：
文献类型：
--
作者：
Shuguang Zhang;Lin Yan;M. Altman;M. Lässle;H. Nugent;F. Frankel;D. Lauffenburger;G. Whitesides
使用合成生物材料的生物表面工程在我们对复杂生物现象的理解方面具有巨大的潜力。我们开发了一个简单的系统，使用自组装寡肽单层和微接触印刷（μCP）的组合来设计生物相关表面。我们设计并合成了两个寡肽，其N-末端含有细胞粘附基序（RADS）n（n=2和3），随后是寡（丙氨酸）接头和C-末端的半胱氨酸残基。半胱氨酸的巯基允许寡肽共价连接到金涂覆的表面上以形成单层。然后，我们使用细胞粘附肽与六乙二醇硫醇盐组合，其抵抗蛋白质和细胞的非特异性吸附，微制造了各种表面图案。所得图案由支持或抑制细胞粘附的区域组成，因此它们能够以明确定义的方式对齐细胞，从而形成特定的细胞阵列和图案。
Biological surface engineering using synthetic biological materials has a great potential for advances in our understanding of complex biological phenomena. We developed a simple system to engineer biologically relevant surfaces using a combination of self-assembling oligopeptide monolayers and microcontact printing (μCP). We designed and synthesized two oligopeptides containing a cell adhesion motif (RADS)n(n=2 and 3) at the N-terminus, followed by an oligo(alanine) linker and a cysteine residue at the C-terminus. The thiol group of cysteine allows the oligopeptides to attach covalently onto a gold-coated surface to form monolayers. We then microfabricated a variety of surface patterns using the cell adhesion peptides in combination with hexa-ethylene glycol thiolate which resist non-specific adsorption of proteins and cells. The resulting patterns consist of areas either supporting or inhibiting cell adhesion, thus they are capable of aligning cells in a well-defined manner, leading to specific cell array and pattern formations.