Primate trabeculectomies with 5-fluorouracil collagen implants.
Primate trabeculectomies with 5-fluorouracil collagen implants.
复制标题
使用 5-氟尿嘧啶胶原蛋白植入物进行灵长类小梁切除术。
DOI：
10.1016/s0002-9394(14)72443-2
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发表时间：
1990
影响因子：
4.2
通讯作者：
Minckler,DS
中科院分区：
文献类型：
--
作者：
Hasty,B;Heuer,DK;Minckler,DS
对4只正常食蟹猴进行双侧小梁切除术。将具有1 mg 5-氟尿嘧啶或生理盐水的高度纯化的牛皮肤胶原植入物（通过随机化、掩蔽分配，每只猴各一个）缝合在巩膜瓣下。在完成所有临床和组织病理学观察之前，不破盲随机化代码。术后6周，5-氟尿嘧啶治疗眼的内部小梁切除术开口比对照眼更深;然而，眼内压无法区分，滤过泡仅存在极轻微差异，每组中有一半有微量或轻度前囊下晶状体变化。在组织学上，多核异物巨细胞反应在所有胶原蛋白植入物中均很明显，但与对照眼相比，5-氟尿嘧啶治疗眼的反应不太剧烈或延迟。胶原蛋白植入物在青光眼滤过手术模型中似乎是一种不良的药物输送系统，因为它会引起肉芽肿性炎症反应。
Bilateral trabeculectomies were performed on four normal cynomolgus monkeys. Highly purified bovine skin collagen implants with either 1 mg of 5-fluorouracil or physiologic saline (one each per monkey by randomized, masked assignment) were sutured under the scleral flaps. The randomization code was not broken until all clinical and histopathologic observations had been completed. Six weeks postoperatively, the 5-fluorouracil–treated eyes had more deeply open internal trabeculectomy ostia than the control eyes; however, the intraocular pressures were indistinguishable, the filtering blebs were only minimally different, and half of each group had trace or mild anterior subcapsular lenticular changes. Histologically, a multinucleated foreign body giant cell reaction was evident in all of the collagen implants, but the 5-fluorouracil–treated eyes had less vigorous or delayed reactions compared to the control eyes. The collagen implant appears to be a poor drug delivery system in this model of glaucoma filtering surgery, because it incites a granulomatous inflammatory reaction.