Simultaneous Microcystis algicidal and microcystin synthesis inhibition by a red pigment prodigiosin
Simultaneous Microcystis algicidal and microcystin synthesis inhibition by a red pigment prodigiosin
复制标题
红色素灵菌红同时抑制微囊藻和微囊藻毒素合成
DOI：
10.1016/j.envpol.2019.113444
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发表时间：
2020-01-01
影响因子：
8.9
通讯作者：
Yang, Fei
中科院分区：
文献类型：
--
作者：
Wei, Jia;Xie, Xian;Yang, Fei
微囊藻水华及其次级代谢产物微囊藻毒素（Microcystis microcystins，MCs）在世界范围内广泛发生，严重破坏了水生生态系统，威胁着公众健康。减少微囊藻水华和MC的技术是迫切需要的。本研究旨在探讨红色素灵菌红素（PG）对铜绿微囊藻（M. aeruginosa）生长和产生MC能力的杀藻和抑制机制。铜绿）。显微镜下计数微囊藻细胞数。采用实时荧光定量聚合酶链反应（qRT-PCR）和酶联免疫吸附试验（ELISA）分别检测微囊藻毒素合成酶B基因（mcy B）的表达和MCs的浓度。PG对M.铜绿假单胞菌FACHB 905在120 h的50%杀藻浓度（LC 50）为0.12 μ g/mL。当M. 0.08 μ g/mL，0.16 μ g/mL，0.32 μ g/mLPG作用于铜绿假单胞菌细胞后，其mcyB的表达量明显增加。铜绿假单胞菌在120 h时分别下调了4.36、8.16和18.51倍。120 h时总MC（TMC）浓度分别为对照的1.66、1.72和5.75倍。PG对M.铜绿假单胞菌感染后，超氧化物歧化酶（SOD）活性先升高后下降，丙二醛（MDA）含量升高，mcyB基因表达下调，MCs合成受到抑制。本研究首次报道了PG可同时裂解微囊藻细胞、下调mcyB的表达并有效抑制MCs的产生，这可能与氧化应激有关，表明PG在调控环境中微囊藻水华和MCs污染方面具有很大的潜力。(C)2019爱思唯尔有限公司版权所有。
Microcystis blooms and their secondary metabolites microcystins (MCs) occurred all over the world, which have damaged aquatic ecosystems and threatened public health. Techniques to reduce the Microcystis blooms and MCs are urgently needed. This study aimed to investigate the algicidal and inhibitory mechanisms of a red pigment prodigiosin (PG) against the growth and MC-producing abilities of Microcystis aeruginosa (M. aeruginosa). The numbers of Microcystis cells were counted under microscope. The expression of microcystin synthase B gene (mcyB) and concentrations of MCs were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme linked immunosorbent assay (ELISA) methods, respectively. The inhibitory effects of PG against M. aeruginosa strain FACHB 905 with 50% algicidal concentration (LC50) at 120 h was 0.12 mu g/mL. When M. aeruginosa cells exposed to 0.08 mu g/mL, 0.16 mu g/mL, 0.32 mu g/mL PG, the expression of mcyB of M. aeruginosa was down-regulated 4.36, 8.16 and 18.51 times lower than that of the control at 120 h. The concentrations of total MC (TMC) also were 1.66, 1.72 and 5.75 times lower than that of the control at 120 h. PG had high algicidal effects against M. aeruginosa, with the activities of superoxide dismutase (SOD) initially increased and then decreased after 72 h, the contents of malondialdehyde (MDA) increase, the expression of mcyB gene down-regulation, and MCs synthesis inhibition. This study was first to report the PG can simultaneously lyse Microcystis cells, down-regulate of mcyB expression and inhibit MCs production effectively probably due to oxidative stress, which indicated PG poses a great potential for regulating Microcystis blooms and MCs pollution in the environment. (C) 2019 Elsevier Ltd. All rights reserved.