Specific p53 mutations predict poor prognosis in oral squamous cell carcinoma.
Specific p53 mutations predict poor prognosis in oral squamous cell carcinoma.
复制标题
DOI：
10.1016/s1368-8375(02)00064-7
复制
发表时间：
2003-02
期刊：
影响因子：
4.8
通讯作者：
Y. Yamazaki;I. Chiba;A. Hirai;C. Sugiura;K. Notani;H. Kashiwazaki;K. Tei;Y. Totsuka;H. Fukuda
中科院分区：
文献类型：
--
作者：
Y. Yamazaki;I. Chiba;A. Hirai;C. Sugiura;K. Notani;H. Kashiwazaki;K. Tei;Y. Totsuka;H. Fukuda
在这项研究中，我们专注于特定区域的p53突变，包括DNA结合表面区域，以阐明p53特定区域内的突变与口腔癌患者临床结局之间的相关性。我们通过聚合酶链反应-单链构象多态性或酵母功能分析法分析了121例新鲜原发性口腔鳞状细胞癌（SCC）中的p53突变。在51/121例（42%）病例中检测到p53突变。p53突变与任何临床病理学参数无关;然而，含有特定p53突变的肿瘤，例如DNA结合表面区域（L2、L3和LSH基序）和保守区域（II-V），比那些区域外突变的肿瘤具有显著较差的预后。此外，局部区域失败，淋巴结转移和随后的远处转移的发生也显着相关的DNA结合表面区域内的突变。这些数据表明，p53的特定突变可能是口腔SCC的重要预后因素。
In this study, we focused on p53 mutations in specific regions, including DNA-binding surface regions, to clarify the correlation between mutations within the specific regions of p53 and clinical outcomes of patients with oral cancers. We analyzed p53 mutations in 121 fresh primary oral squamous cell carcinomas (SCCs) by polymerase chain reaction-single-strand conformation polymorphism or a yeast functional assay. p53 mutations were detected in 51/121 (42%) cases. Mutation of p53 was not associated with any clinicopathological parameters; however, tumors containing specific p53 mutations, e.g. DNA-binding surface regions (L2, L3 and the LSH motif) and conserved regions (II–V), had significantly poorer prognoses than tumors with mutations outside of those regions. Moreover, locoregional failure, lymph node metastasis and the occurrence of subsequent distant metastasis were also significantly associated with mutations within DNA-binding surface regions. These data indicate that specific mutations of p53 could be important prognostic factors in oral SCCs.