Study of human salivary proline-rich proteins interaction with food tannins
Study of human salivary proline-rich proteins interaction with food tannins
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DOI：
10.1016/j.foodchem.2017.09.063
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发表时间：
2018-03-15
期刊：
影响因子：
8.8
通讯作者：
de Freitas, Victor
中科院分区：
文献类型：
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作者：
Soares, Susana;Garcia-Estevez, Ignacio;de Freitas, Victor
本工作采用饱和转移差分核磁共振、等温微量热法和分子动力学模拟等方法研究了碱性、糖基化和酸性富含脯氨酸的蛋白质(bPRPS、gPRPs、aPRPs)和P-B肽与几种具有代表性的食品单宁[原花青素B2、原花青素B2 3‘-O-没食子酸酯(B2G)和原花青素三聚体(儿茶素-4-8-儿茶素-4-8-儿茶素)]之间的相互作用。结果表明，P-B肽是亲和力较高的唾液蛋白，而aPRPs与所研究的原花青素亲和力较低。此外，B2G是与所有SP亲和力较高的原花青素。在所有相互作用中都存在疏水和氢键，但主要驱动力依赖于原花青素-SP对。此外，其附近的脯氨酸簇或残基被确定为蛋白质与原花青素相互作用的可能位置。在原花青素结合后，bPRP和APRP的构象发生了显著的变化，而P-B肽没有表现出任何结构重排。
In this work, saturation transfer difference-NMR, isothermal microcalorimetry and molecular dynamics simulations have been used to study the individual interactions between basic, glycosylated and acidic proline-rich proteins (bPRPS, gPRPs, aPRPs) and P-B peptide with some representative food tannins [procyanidin B2, procyanidin B2 3'-O-gallate (B2g) and procyanidin trimer (catechin-4-8-catechin-4-8-catechin)]. Results showed that P-B peptide was in general the salivary protein (SP) with higher affinity whereas aPRPs showed lower affinity to the studied procyanidins. Moreover, B2g was the procyanidin with higher affinity for all SP. Hydrophobic and hydrogen bonds were present in all interactions but the major driving force depended on the procyanidin-SP pair. Furthermore, proline clusters or residues in their vicinity were identified as the probable sites of proteins for interaction with procyanidins. For bPRP and aPRP a significant change to less extended conformations was observed, while P-B peptide did not display any structural rearrangement upon procyanidins binding.