Molecular identification of grass carp igfbp2 and the effect of glucose, insulin, and glucagon on igfbp2 mRNA expression
Molecular identification of grass carp igfbp2 and the effect of glucose, insulin, and glucagon on igfbp2 mRNA expression
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草鱼igfbp2的分子鉴定及葡萄糖、胰岛素、胰高血糖素对igfbp2 mRNA表达的影响
DOI：
10.1007/s10695-020-00804-w
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发表时间：
2020-04-23
影响因子：
2.9
通讯作者：
Nie，Guoxing
中科院分区：
文献类型：
--
作者：
Yang，Guokun;Zhao，Wenli;Nie，Guoxing
生长激素（GH）/胰岛素样生长因子（IGFs）系统在生长调节中具有重要作用。在这个系统中，IGFBPs在IGF功能中起着至关重要的调节作用。IGFBP 2是IGFBP家族的成员之一，它可以与IGF结合，调节IGF的功能，从而调节发育和生长。此外，IGFBP 2在细胞增殖和代谢中显示出关键的调节功能。本研究从草鱼（Ctenopharyngodon idellus）肝脏中克隆了igfbp 2基因。草鱼igfbp 2基因的开放阅读框长834 bp，编码277个氨基酸。组织分布结果表明，igfbp 2在草鱼体内多种组织中均有表达，且在肝脏中表达量较高。在OGTT中，用葡萄糖处理6 h后，肝脏和大脑中的igfbp 2表达显著降低。在体外实验中，胰岛素处理草鱼原代肝细胞6和12 h后，igfbp 2的表达受到显著抑制。此外，在草鱼原代肝细胞中，胰高血糖素孵育显著增加igfbp 2的表达，并呈剂量依赖性。据我们所知，这是草鱼Igfbp 2的首次报道。这些结果将为草鱼Igfbp 2的深入研究提供基础。
The GH (growth hormone)/IGFs (insulin-like growth factors) system has an important function in the regulation of growth. In this system, IGFBPs play a crucial regulatory role in IGF functions. As a member of the IGFBP family, IGFBP2 can bind to IGF and regulate IGF functions to regulate development and growth. In addition, IGFBP2 shows key regulatory functions in cell proliferation and metabolism. In this study, the igfbp2 gene was cloned from grass carp (Ctenopharyngodon idellus) liver. The ORF of grass carp igfbp2 is 834 bp long and encodes 277 amino acids. The tissue distribution results showed that igfbp2 is expressed in multiple tissues in grass carp and has a high expression level in the liver. In the OGTT, igfbp2 expression was significantly decreased in the liver and brain after 6 h of treatment with glucose. In vitro, igfbp2 expression in grass carp's primary hepatocytes was significantly suppressed by insulin after treatment for 6 and 12 h. Moreover, igfbp2 expression was markedly increased in a dose-dependent manner with glucagon incubation in grass carp's primary hepatocytes. To the best of our knowledge, this is the first report about Igfbp2 in grass carp. These results will provide a basis for the in-depth study of grass carp Igfbp2.