Continuous dielectrophoretic particle separation using a microfluidic device with 3D electrodes and vaulted obstacles
Continuous dielectrophoretic particle separation using a microfluidic device with 3D electrodes and vaulted obstacles
复制标题
使用具有 3D 电极和拱形障碍物的微流体装置进行连续介电泳颗粒分离
DOI：
10.1002/elps.201400565
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发表时间：
2015-08-01
期刊：
影响因子：
2.9
通讯作者：
Jiang, Hongyuan
中科院分区：
文献类型：
--
作者：
Jia, Yankai;Ren, Yukun;Jiang, Hongyuan
本文报道了一种微流控分离装置，该装置结合了3D电极和拱形障碍物，以连续地将经历强正介电泳（DEP）的颗粒与经历弱正介电泳（DEP）的颗粒或经历负介电泳的颗粒分离。分离是通过首先通过流体动力学聚焦流将颗粒混合物聚焦成窄流，然后通过AC DEP将它们偏离到不同的出口来实现的。拱形障碍物通过增加电场的不均匀性和影响颗粒在受DEP强烈影响的区域中移动来促进分离。3D电极产生空间非均匀电场并将DEP效应扩展到沟道高度。通过数值模拟研究了障碍物对电场分布和粒子运动轨迹的影响，优化了障碍物的高度，并与实验结果进行了比较。通过使用正DEP和负DEP在不同流速下将25 μm金包被颗粒与10 μm颗粒分离，以及仅使用正DEP将25 μm金包被颗粒与酵母细胞分离，来评估装置的性能。实验观察表明，与数值模拟结果的合理协议。
This paper reports a microfluidic separation device combining 3D electrodes and vaulted obstacles to continuously separate particles experiencing strong positive dielectrophoresis (DEP) from particles experiencing weak positive DEP, or from particles experiencing negative DEP. The separation is achieved by first focusing the particle mixture into a narrow stream by a hydrodynamic focusing flow, and then deviating them into different outlets by AC DEP. The vaulted obstacles facilitate the separation by both increasing the non‐uniformity of the electric field, and influencing the particles to move in regions strongly affected by DEP. The 3D electrodes give rise to a spatially non‐uniform electric field and extend DEP effect to the channel height. Numerical simulations are performed to investigate the effects of the obstacles on electric field distribution and particle trajectories so as to optimize the obstacle height and compare with the experimental results. The performance of the device is assessed by separating 25 μm gold‐coated particles from 10 μm particles in different flow rates by positive DEP and negative DEP, and also separating 25 μm gold‐coated particles from yeast cells using only positive DEP. The experimental observation shows a reasonable agreement with numerical simulation results.